Research Institute for Medical Sciences, College of Medicine, Inha University, Incheon 22212, Korea,
College of Electrical Engineering, Korea University, Seoul 02841, Korea.
Int J Nanomedicine. 2019 Mar 7;14:1737-1751. doi: 10.2147/IJN.S192277. eCollection 2019.
Zinc oxide (ZnO) nanoparticles and their networks have been developed for use in various applications such as gas sensors and semiconductors.
In this study, their antibacterial activity against under dual ultraviolet (UV) irradiation for disinfection was investigated.
ZnO nanoparticles were synthesized and immobilized onto silicon (Si) wafers by self-assembly. The physicochemical properties and antibacterial activity of ZnO nanoparticles and their networks were evaluated. Gene ontology was analyzed and toxicity levels were also monitored.
Synthesized ZnO nanoparticles were spherical nanocrystals (<100 nm; Zn, 47%; O, 53%) that formed macro-mesoporous three-dimensional nanostructures on Si wafers in a concentration-dependent manner. ZnO nanoparticles and their networks on Si wafers had an excellent antibacterial activity against under dual UV irradiation (>3log CFU/mL). Specifically, arrayed ZnO nanoparticle networks showed superior activity compared with free synthesized ZnO nanoparticles. Oxidative stress-responsive proteins in were identified and categorized, which indicated antibacterial activity. Synthesized ZnO nanoparticles were less cytotoxic in HaCaT with an IC50 of 6.632 mg/mL, but phototoxic in Balb/c 3T3.
The results suggested that ZnO nanoparticles and their networks can be promising photocatalytic antibiotics for use in next-generation disinfection systems. Their application could also be extended to industrial and clinical use as effective and safe photocatalytic antibiotics.
氧化锌（ZnO）纳米粒子及其网络已被开发用于各种应用，如气体传感器和半导体。
本研究旨在探讨其在双紫外（UV）辐射下作为消毒剂对 的抗菌活性。
通过自组装将 ZnO 纳米粒子合成并固定在硅（Si）晶片上。评估了 ZnO 纳米粒子及其网络的物理化学性质和抗菌活性。进行了基因本体分析，并监测了毒性水平。
合成的 ZnO 纳米粒子为球形纳米晶体（<100nm；Zn，47%；O，53%），以浓度依赖的方式在 Si 晶片上形成了大-中孔三维纳米结构。Si 晶片上的 ZnO 纳米粒子及其网络在双 UV 照射下对 具有极好的抗菌活性（>3log CFU/mL）。具体而言，排列的 ZnO 纳米粒子网络显示出比游离合成的 ZnO 纳米粒子更高的活性。鉴定并分类了 中的氧化应激反应蛋白，表明了其抗菌活性。合成的 ZnO 纳米粒子在 HaCaT 中的细胞毒性较小，IC50 为 6.632mg/mL，但在 Balb/c 3T3 中具有光毒性。
结果表明，ZnO 纳米粒子及其网络可以作为有前途的光催化抗生素，用于下一代消毒系统。它们的应用还可以扩展到工业和临床应用，作为有效和安全的光催化抗生素。
