Chambers J K, Matsumoto I, Shibahara T, Haritani M, Nakayama H, Uchida K
Laboratory of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, the University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.
National Institute of Animal Health, National Agricultural and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki, Japan.
J Comp Pathol. 2019 Feb;167:41-45. doi: 10.1016/j.jcpa.2018.12.002. Epub 2019 Jan 14.
Twenty-two newborn puppies that did not receive colostrum exhibited acute respiratory signs and died at a breeding facility. Pathological examinations were performed on four of the puppies. At necropsy examination, the lungs were firm and mottled dark red, consistent with acute bronchopneumonia. Histopathologically, there was marked infiltration of neutrophils and macrophages into the bronchi and alveoli, and gram-negative coccobacilli were attached diffusely to the cilia of bronchial mucosa. Immunohistochemistry for Bordetella bronchiseptica antigen revealed positive labelling of the bacterial agents. On electron microscopy, a large number of coccobacilli were observed attaching to the cilia of bronchial epithelial cells. Real-time polymerase chain reaction amplified a B. bronchiseptica gene from the affected lung tissue. Based on these findings, the four puppies were diagnosed with fatal B. bronchiseptica bronchopneumonia.
22只未摄入初乳的新生幼犬在一个繁育场所出现急性呼吸道症状并死亡。对其中4只幼犬进行了病理检查。尸检时，肺质地坚实，呈斑驳的暗红色，符合急性支气管肺炎表现。组织病理学检查显示，支气管和肺泡中有大量中性粒细胞和巨噬细胞浸润，革兰氏阴性球杆菌弥漫性附着于支气管黏膜纤毛上。支气管败血波氏杆菌抗原的免疫组织化学检查显示病原体呈阳性标记。电子显微镜检查发现大量球杆菌附着于支气管上皮细胞的纤毛上。实时聚合酶链反应从受影响的肺组织中扩增出支气管败血波氏杆菌基因。基于这些发现，这4只幼犬被诊断为致命性支气管败血波氏杆菌支气管肺炎。
