Tsinghua-Peking Joint Center for Life Sciences, School of Medicine, Tsinghua University, 100084, Beijing, People's Republic of China.
Beijing Advanced Innovation Center for Structural Biology, School of Medicine, Tsinghua University, 100084, Beijing, People's Republic of China.
Nat Commun. 2019 Mar 22;10(1):1324. doi: 10.1038/s41467-019-09256-0.
Zika virus (ZIKV) is a mosquito-borne flavivirus that predominantly circulates between humans and Aedes mosquitoes. Clinical studies have shown that Zika viruria in patients persists for an extended period, and results in infectious virions being excreted. Here, we demonstrate that Aedes mosquitoes are permissive to ZIKV infection when breeding in urine or sewage containing low concentrations of ZIKV. Mosquito larvae and pupae, including from field Aedes aegypti can acquire ZIKV from contaminated aquatic systems, resulting in ZIKV infection of adult females. Adult mosquitoes can transmit infectious virions to susceptible type I/II interferon receptor-deficient (ifnagr-/-) C57BL/6 (AG6) mice. Furthermore, ZIKV viruria from infected AG6 mice can causes mosquito infection during the aquatic life stages. Our studies suggest that infectious urine could be a natural ZIKV source, which is potentially transmissible to mosquitoes when breeding in an aquatic environment.
Zika 病毒（ZIKV）是一种蚊媒黄病毒，主要在人类和埃及伊蚊之间循环。临床研究表明，患者的 Zika 病毒尿持续时间较长，导致传染性病毒颗粒排出。在这里，我们证明当在含有低浓度 ZIKV 的尿液或污水中繁殖时，埃及伊蚊对 ZIKV 感染是允许的。蚊子幼虫和蛹，包括来自野外的埃及伊蚊，可以从受污染的水生系统中获得 ZIKV，导致成年雌性蚊子感染 ZIKV。成年蚊子可以将传染性病毒颗粒传播给易感的 I/II 型干扰素受体缺陷型（ifnagr-/-）C57BL/6（AG6）小鼠。此外，来自感染的 AG6 小鼠的 Zika 病毒尿可导致在水生生活阶段感染蚊子。我们的研究表明，传染性尿液可能是 Zika 病毒的天然来源，当蚊子在水生环境中繁殖时，它可能具有传染性。
