Department of Physics, Colorado School of Mines, Golden, Colorado, United States of America.
Department of Chemistry, Colorado School of Mines, Golden, Colorado, United States of America.
PLoS One. 2019 Mar 26;14(3):e0214411. doi: 10.1371/journal.pone.0214411. eCollection 2019.
Antimicrobial biopolymers provide a biodegradable, sustainable, safe, and cheap approach to drug delivery and wound dressing to control bacterial infection and improve wound healing respectively. Here, we report a one-step method of making antimicrobial alginate polymer from sodium alginate and aqueous extract of Wakame using antibiotic aminoglycosides. Thin layer chromatography of commercially available sodium alginate and Wakame extract showed similar oligosaccharide profiles. Screening of six aminoglycosides showed that kanamycin disulfate and neomycin sulfate produces the highest amount of biopolymer; however, kanamycin disulfate produces the most malleable and form fitting biopolymer. Image texture analysis of biopolymers showed similar quantification parameters for all the six aminoglycosides. Weight of alginate polymer as a function of aminoglycoside concentration follows a growth model of prion protein, consistent with the aggregating nature of both processes. Slow release of antibiotics and the resulting zone of inhibition against E. coli DH5α were observed by agar well diffusion assay. Inexpensive method of production and slow release of antibiotics will enable diverse applications of antimicrobial alginate biopolymer reported in this paper.
抗菌生物聚合物为药物输送和伤口敷料提供了一种可生物降解、可持续、安全且廉价的方法，分别用于控制细菌感染和促进伤口愈合。在这里，我们报告了一种使用抗生素氨基糖苷类药物从海藻酸钠和裙带菜水提物一步制备抗菌海藻酸钠聚合物的方法。市售海藻酸钠和裙带菜提取物的薄层层析显示出相似的低聚糖图谱。对六种氨基糖苷类药物的筛选表明，硫酸卡那霉素和硫酸新霉素产生的生物聚合物最多；然而，硫酸卡那霉素产生的生物聚合物最具延展性和适应性。生物聚合物的图像纹理分析显示，所有六种氨基糖苷类药物的定量参数相似。海藻酸钠聚合物的重量作为氨基糖苷类药物浓度的函数遵循普里昂蛋白的生长模型，与两种过程的聚集性质一致。琼脂孔扩散法观察到抗生素的缓慢释放和对大肠杆菌 DH5α 的抑制区。这种生产方法廉价且抗生素缓慢释放，将使本文报道的抗菌海藻酸钠生物聚合物得到广泛应用。
