粪肠球菌性信息素cAM373的分离与结构
Isolation and structure of the Streptococcus faecalis sex pheromone, cAM373.
作者信息
Mori M, Tanaka H, Sakagami Y, Isogai A, Fujino M, Kitada C, White B A, An F Y, Clewell D B, Suzuki A
出版信息
FEBS Lett. 1986 Sep 29;206(1):69-72. doi: 10.1016/0014-5793(86)81342-4.
The Streptococcus faecalis sex pheromone, cAM373, which induces a mating response of donor cells harboring plasmid pAM373 and is also produced by Staphylococcus aureus, was isolated and its structure determined. Supernatant from an overnight culture of a recipient strain was subjected to successive purification procedures, and 4.4 micrograms cAM373 was obtained. The isolated pheromone showed activity at a concentration as low as 5 X 10(-11) M. Sequence analysis indicated that cAM373 was a heptapeptide, H-Ala-Ile-Phe-Ile-Leu-Ala-Ser-OH, and that its Mr was 733. A synthetic replicate of the peptide showed the same biological activity and chromatographic behavior as the native cAM373.
粪肠球菌性信息素cAM373可诱导携带质粒pAM373的供体细胞产生交配反应，金黄色葡萄球菌也能产生该物质。现已分离出该信息素并确定了其结构。对受体菌株过夜培养物的上清液进行连续纯化，获得了4.4微克的cAM373。分离出的信息素在低至5×10⁻¹¹ M的浓度下仍具有活性。序列分析表明，cAM373是一种七肽，即H-Ala-Ile-Phe-Ile-Leu-Ala-Ser-OH，其相对分子质量为733。该肽的合成复制品与天然cAM373具有相同的生物活性和色谱行为。
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