Jiang Guozhan, Johnston Brian, Townrow David E, Radecka Iza, Koller Martin, Chaber Paweł, Adamus Grażyna, Kowalczuk Marek
School of Biology Chemistry and Forensic Science, University of Wolverhampton, Wolverhampton WV11LY, UK.
C/o Institute of Chemistry, Office of Research Management and Service, University of Graz, NAWI Graz, Heinrichstrasse 28/III, 8010 Graz, Austria.
Polymers (Basel). 2018 Jul 3;10(7):731. doi: 10.3390/polym10070731.
An economically viable method to extract polyhydroxyalkanoates (PHAs) from cells is desirable for this biodegradable polymer of potential biomedical applications. In this work, two non-chlorinated solvents, cyclohexanone and γ-butyrolactone, were examined for extracting PHA produced by the bacterial strain H16 cultivated on vegetable oil as a sole carbon source. The PHA produced was determined as a poly(3-hydroxybutyrate) (PHB) homopolyester. The extraction kinetics of the two solvents was determined using gel permeation chromatography (GPC). When cyclohexanone was used as the extraction solvent at 120 °C in 3 min, 95% of the PHB was recovered from the cells with a similar purity to that extracted using chloroform. With a decrease in temperature, the recovery yield decreased. At the same temperatures, the recovery yield of γ-butyrolactone was significantly lower. The effect of the two solvents on the quality of the extracted PHB was also examined using GPC and elemental analysis. The molar mass and dispersity of the obtained polymer were similar to that extracted using chloroform, while the nitrogen content of the PHB extracted using the two new solvents was slightly higher. In a nutshell, cyclohexanone in particular was identified as an expedient candidate to efficiently drive novel, sustainable PHA extraction processes.
对于这种具有潜在生物医学应用价值的可生物降解聚合物，需要一种经济可行的方法从细胞中提取聚羟基脂肪酸酯（PHA）。在这项工作中，研究了两种非氯化溶剂环己酮和γ-丁内酯，用于提取以植物油为唯一碳源培养的细菌菌株H16产生的PHA。所产生的PHA被确定为聚（3-羟基丁酸酯）（PHB）均聚物。使用凝胶渗透色谱法（GPC）测定了两种溶剂的提取动力学。当在120°C下使用环己酮作为提取溶剂3分钟时，95%的PHB从细胞中回收，其纯度与使用氯仿提取的纯度相似。随着温度降低，回收率下降。在相同温度下，γ-丁内酯的回收率显著较低。还使用GPC和元素分析研究了两种溶剂对提取的PHB质量的影响。所得聚合物的摩尔质量和分散度与使用氯仿提取的相似，而使用两种新溶剂提取的PHB的氮含量略高。简而言之，特别是环己酮被确定为有效推动新型可持续PHA提取工艺的合适候选物。
