Kozlowska Justyna, Stachowiak Natalia, Sionkowska Alina
Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarina 7, 87-100 Torun, Poland.
Polymers (Basel). 2018 Apr 19;10(4):456. doi: 10.3390/polym10040456.
The objective of this study was to develop three-dimensional collagen/gelatin/hydroxyethyl cellulose composites in combination with gelatin or collagen-gelatin loaded microspheres. Microspheres were prepared by an emulsification/crosslinking method. A 1-Ethyl3-(3dimethyl-aminopropyl)-carbodiimide (EDC) and -hydroxysuccinimide (NHS) mixture were used as a crosslinking agent for the obtained materials. The structure of the materials was studied using scanning electron microscopy (SEM) and infrared spectroscopy. Moreover, a (pot marigold) flower extract release profile of the microsphere-loaded matrices was assessed in vitro. Additionally, porosity, density, stability, swelling and mechanical properties were tested. On the basis of SEM images, the microspheres exhibited a spherical shape and were irregularly dispersed in the polymer matrix. However, it was found that the addition of microparticles to obtained materials did not significantly change their microstructure. We observed a slight decrease in the swelling properties of matrices and an increase in values of Young's modulus. Significantly, the addition of microspheres to the polymer matrices led to improved loading capacity of materials and release performance of flower extract. This makes the collagen/gelatin/hydroxyethyl cellulose composites containing microspheres a promising and suitable vehicle for biomedical, dermatological, or cosmetic applications.
本研究的目的是开发三维胶原蛋白/明胶/羟乙基纤维素复合材料，并结合负载明胶或胶原-明胶的微球。微球通过乳化/交联法制备。使用1-乙基-3-(3-二甲基氨基丙基)-碳二亚胺(EDC)和N-羟基琥珀酰亚胺(NHS)混合物作为所得材料的交联剂。使用扫描电子显微镜(SEM)和红外光谱研究材料的结构。此外，在体外评估了负载微球的基质中万寿菊提取物的释放曲线。另外，测试了孔隙率、密度、稳定性、溶胀和力学性能。基于SEM图像，微球呈球形，不规则地分散在聚合物基质中。然而，发现向所得材料中添加微粒并没有显著改变其微观结构。我们观察到基质的溶胀性能略有下降，杨氏模量值增加。值得注意的是，向聚合物基质中添加微球导致材料的负载能力和万寿菊提取物的释放性能得到改善。这使得含微球的胶原蛋白/明胶/羟乙基纤维素复合材料成为生物医学、皮肤病学或化妆品应用中有前景且合适的载体。
