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核苷酸结合富含亮氨酸重复序列基因的过表达赋予水稻广谱抗病性。

Overexpression of Nucleotide-Binding and Leucine-Rich Repeat Genes and () Confers Broad-Spectrum Disease Resistance in Rice.

作者信息

Li Zhaowu, Huang Jianzhong, Wang Zhangying, Meng Fen, Zhang Siyuan, Wu Xiaoqiu, Zhang Zhihong, Gao Zhiyong

机构信息

State Key Laboratory of Hybrid Rice, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, College of Life Sciences, Wuhan University, Wuhan, China.

出版信息

Front Plant Sci. 2019 Apr 5;10:417. doi: 10.3389/fpls.2019.00417. eCollection 2019.

DOI:10.3389/fpls.2019.00417
PMID:31024591
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6459959/
Abstract

The nucleotide-binding domain leucine-rich repeat (NLR) immune receptors play important roles in innate plant immunity. The activation of NLRs is specifically induced by their cognate effectors released from pathogens. Autoactive NLRs are expected to confer broad-spectrum resistance because they do not need cognate effectors to activate their immune responses. In this study, we demonstrated that the genes and () from were autoactive in and conferred broad-spectrum resistance to fungal pathogen , bacterial pathogen (), and pest brown planthopper (BPH, Stål). These results revealed that interfamily transfer of dicot NLRs to monocot species could be functional. The transgenic plants displayed early and strong induction of reactive oxygen species (ROS), callose deposition, and expression of defense-related genes after challenged with . The transcriptome analysis showed that the expressions of some defense-related genes were primed to adapt the transformed autoactive NLRs in the transgenic plants. This study indicates that autoactive NLRs are a promising resource for breeding crops with broad-spectrum resistance and provides new insights for engineering disease resistance.

摘要

核苷酸结合结构域富含亮氨酸重复序列（NLR）免疫受体在植物先天免疫中发挥重要作用。NLR的激活是由病原体释放的同源效应子特异性诱导的。预期自激活NLR可赋予广谱抗性，因为它们不需要同源效应子来激活其免疫反应。在本研究中，我们证明了来自[植物名称未提及]的基因[基因名称未提及]和[基因名称未提及]在[植物名称未提及]中具有自激活能力，并对真菌病原体[病原体名称未提及]、细菌病原体[病原体名称未提及]（[细菌病原体名称未提及]）和害虫褐飞虱（BPH，[褐飞虱学名未提及]）具有广谱抗性。这些结果表明双子叶植物NLR向单子叶植物的跨家族转移可能具有功能。转基因植物在用[病原体名称未提及]攻击后表现出活性氧（ROS）的早期强烈诱导、胼胝质沉积以及防御相关基因的表达。转录组分析表明，一些防御相关基因的表达已做好准备以适应转基因植物中转化的自激活NLR。本研究表明自激活NLR是培育具有广谱抗性作物的有前途的资源，并为工程抗病性提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d8/6459959/25f57168d840/fpls-10-00417-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d8/6459959/2a4287f33d99/fpls-10-00417-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d8/6459959/1007cb874010/fpls-10-00417-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d8/6459959/5e277f1c56ae/fpls-10-00417-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d8/6459959/a998c6198b19/fpls-10-00417-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d8/6459959/ec1b6b3e2d72/fpls-10-00417-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d8/6459959/3c189778626b/fpls-10-00417-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d8/6459959/75a3be7b76db/fpls-10-00417-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d8/6459959/b10b9243a480/fpls-10-00417-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d8/6459959/25f57168d840/fpls-10-00417-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d8/6459959/2a4287f33d99/fpls-10-00417-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d8/6459959/1007cb874010/fpls-10-00417-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d8/6459959/5e277f1c56ae/fpls-10-00417-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d8/6459959/a998c6198b19/fpls-10-00417-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d8/6459959/ec1b6b3e2d72/fpls-10-00417-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d8/6459959/3c189778626b/fpls-10-00417-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d8/6459959/75a3be7b76db/fpls-10-00417-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d8/6459959/b10b9243a480/fpls-10-00417-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64d8/6459959/25f57168d840/fpls-10-00417-g009.jpg

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