通过多重线性扩增进行CEL-Seq2单细胞RNA测序

CEL-Seq2-Single-Cell RNA Sequencing by Multiplexed Linear Amplification.

作者信息

Yanai Itai, Hashimshony Tamar

机构信息

Institute for Computational Medicine, NYU School of Medicine, New York, NY, USA.

Lokey Interdisciplinary Center for Life Sciences and Engineering, Technion-Israel Institute of Technology, Haifa, Israel.

出版信息

Methods Mol Biol. 2019;1979:45-56. doi: 10.1007/978-1-4939-9240-9_4.

DOI:10.1007/978-1-4939-9240-9_4

PMID:31028631

Abstract

Single-cell RNA sequencing has revolutionized the way we look at cell populations. Of the methods available, CEL-Seq was the first to use linear RNA amplification. With early barcoding and 3' sequencing, it is sensitive, cost-effective and easy to perform. Here we describe a protocol for performing CEL-Seq2 on sorted cells, which can be performed without any special equipment.

摘要

单细胞RNA测序彻底改变了我们看待细胞群体的方式。在现有的方法中，CEL-Seq是最早使用线性RNA扩增的方法。它具有早期条形码和3'测序技术，灵敏、经济高效且易于操作。在此，我们描述了一种在分选细胞上进行CEL-Seq2的方案，该方案无需任何特殊设备即可实施。