基于多次退火和加尾的定量单细胞RNA测序（MATQ-Seq）进行单细胞RNA测序

Single-Cell RNA-Seq by Multiple Annealing and Tailing-Based Quantitative Single-Cell RNA-Seq (MATQ-Seq).

作者信息

Sheng Kuanwei, Zong Chenghang

机构信息

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.

出版信息

Methods Mol Biol. 2019;1979:57-71. doi: 10.1007/978-1-4939-9240-9_5.

DOI:10.1007/978-1-4939-9240-9_5

PMID:31028632

Abstract

Single-cell technologies have emerged as advanced tools to study various biological processes that demand the single cell resolution. To detect subtle heterogeneity in the transcriptome, high accuracy and sensitivity are still desired for single-cell RNA-seq. We describe here multiple annealing and dC-tailing-based quantitative single-cell RNA-seq (MATQ-seq) with ~90% capture efficiency. In addition, MATQ-seq is a total RNA assay allowing for detection of nonpolyadenylated transcripts.

摘要

单细胞技术已成为研究各种需要单细胞分辨率的生物过程的先进工具。为了检测转录组中的细微异质性，单细胞RNA测序仍需要高精度和高灵敏度。我们在此描述了基于多次退火和dC加尾的定量单细胞RNA测序（MATQ-seq），其捕获效率约为90%。此外，MATQ-seq是一种全RNA分析方法，能够检测非聚腺苷酸化转录本。