Srivastava K C
Prostaglandins Leukot Med. 1986 Dec;25(2-3):187-98. doi: 10.1016/0262-1746(86)90065-x.
Aqueous ginger extract was extracted in three organic solvents viz., n-hexane, chloroform and ethyl acetate with increasing polarity. The extracted materials from these solvents reduced platelet thromboxane formation from exogenous arachidonate (AA) and also inhibited platelet aggregation induced by AA, epinephrine, ADP and collagen; in this respect they were most effective against AA-induced aggregation. The extracted material in n-hexane was further resolved by thin-layer chromatography into various fractions some of which were effective in inhibiting platelet thromboxane formation and platelet aggregation. Aqueous ginger extract reduced the formation of TxB2 from AA-labelled platelets without showing effects on platelet phospholipase activity. Thromboxane formation in labelled platelets on activation with calcium ionophore A23187 was reduced by ginger components, isolated from two TLC bands, in a dose-dependent manner (10-100 ug/500 ml). At the higher dose lipoxygenase products were also reduced. Interestingly the incorporation of AA into platelet phospholipids increased in platelets treated with aqueous ginger extract.
水提姜提取物用三种极性递增的有机溶剂即正己烷、氯仿和乙酸乙酯进行提取。从这些溶剂中提取的物质可减少外源性花生四烯酸（AA）诱导的血小板血栓素生成，还能抑制由AA、肾上腺素、ADP和胶原诱导的血小板聚集；在这方面，它们对AA诱导的聚集最为有效。正己烷提取物通过薄层色谱进一步分离成不同组分，其中一些组分在抑制血小板血栓素生成和血小板聚集方面有效。水提姜提取物可减少AA标记血小板中TXB2的生成，而对血小板磷脂酶活性无影响。从两个薄层色谱条带分离得到的姜成分，以剂量依赖方式（10 - 100微克/500毫升）减少了用钙离子载体A23187激活的标记血小板中的血栓素生成。在较高剂量时，脂氧合酶产物也减少。有趣的是，用水提姜提取物处理的血小板中，AA掺入血小板磷脂的量增加。
