Iqbal Muhammad, Raja Naveed Iqbal, Mashwani Zia-Ur-Rehman, Wattoo Feroza Hamid, Hussain Mubashir, Ejaz Muhammad, Saira Hafiza
Department of Botany, PMAS Arid Agriculture University, Rawalpindi, Pakistan.
Department of Biochemistry, PMAS Arid Agriculture University, Rawalpindi, Pakistan.
IET Nanobiotechnol. 2019 Apr;13(2):230-236. doi: 10.1049/iet-nbt.2018.5041.
The present study was designed to check the role of silver nanoparticles (AgNPs) on physiological, biochemical parameters and antioxidants of wheat (Triticum aestivum L.) under heat stress. Plant extract of Moringa oleifera was used for AgNPs synthesis followed by characterization through UV-Vis spectroscopy, SEM, XRD and Zeta analyser. Heat stress was applied in range of 35-40°C for 3 hrs/ day for 3 days to wheat plants at trifoliate stage. Heat stress decreased the RWC (13.2%), MSI (16.3%), chl a (5.2%), chl b (4.1%) and TCCs (9.9%). Wheat plants treated with AgNPs showed significant increase in RWC (12.2%), MSI (26.5%), chl a (10%), chl b (16.4%), TCCs (19%), TPC (2.4%), TFC (2.5%), TASC (2.5%), SOD (1.3%), POX (1.5%), CAT (1.8%), APX (1.2%) and GPX (1.4%), under heat stress. Lower concentration of AgNPs (50 mg/l) decreased the sugar (5.8%) and proline contents (4%), while increase was observed in higher AgNPs concentrations. Overall, AgNPs treatment enhanced thermo-tolerance in wheat plants, but the mechanism of AgNPs action needs further investigation at genome and proteome level in wheat plants under heat stress.
本研究旨在检测银纳米颗粒（AgNPs）对热胁迫下小麦（Triticum aestivum L.）生理、生化参数及抗氧化剂的作用。采用辣木植物提取物合成AgNPs，随后通过紫外可见光谱、扫描电子显微镜、X射线衍射和zeta分析仪进行表征。在三叶期对小麦植株施加35 - 40°C的热胁迫，每天处理3小时，持续3天。热胁迫使相对含水量（RWC）降低了13.2%、膜稳定性指数（MSI）降低了16.3%、叶绿素a（chl a）降低了5.2%、叶绿素b（chl b）降低了4.1%以及总碳水化合物含量（TCCs）降低了9.9%。在热胁迫下，用AgNPs处理的小麦植株的相对含水量显著增加了12.2%、膜稳定性指数增加了26.5%、叶绿素a增加了10%、叶绿素b增加了16.4%、总碳水化合物含量增加了19%、总酚含量（TPC）增加了2.4%、总黄酮含量（TFC）增加了2.5%、总抗氧化能力（TASC）增加了2.5%、超氧化物歧化酶（SOD）增加了1.3%、过氧化物酶（POX）增加了1.5%、过氧化氢酶（CAT）增加了1.8%、抗坏血酸过氧化物酶（APX）增加了1.2%以及谷胱甘肽过氧化物酶（GPX）增加了1.4%。较低浓度的AgNPs（50 mg/l）降低了糖分含量（5.8%）和脯氨酸含量（4%），而在较高浓度的AgNPs中观察到含量增加。总体而言，AgNPs处理增强了小麦植株的耐热性，但在热胁迫下小麦植株中AgNPs的作用机制需要在基因组和蛋白质组水平上进一步研究。
