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Multiplex Detection of Different Magnetic Beads Using Frequency Scanning in Magnetic Frequency Mixing Technique.

作者信息

Achtsnicht Stefan, Pourshahidi Ali Mohammad, Offenhäusser Andreas, Krause Hans-Joachim

机构信息

Institute of Complex Systems Bioelectronics (ICS-8), Forschungszentrum Jülich, 52425 Jülich, Germany.

RWTH Aachen University, 52062 Aachen, Germany.

出版信息

Sensors (Basel). 2019 Jun 7;19(11):2599. doi: 10.3390/s19112599.


DOI:10.3390/s19112599
PMID:31181672
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6603599/
Abstract

In modern bioanalytical methods, it is often desired to detect several targets in one sample within one measurement. Immunological methods including those that use superparamagnetic beads are an important group of techniques for these applications. The goal of this work is to investigate the feasibility of simultaneously detecting different superparamagnetic beads acting as markers using the magnetic frequency mixing technique. The frequency of the magnetic excitation field is scanned while the lower driving frequency is kept constant. Due to the particles' nonlinear magnetization, mixing frequencies are generated. To record their amplitude and phase information, a direct digitization of the pickup-coil's signal with subsequent Fast Fourier Transformation is performed. By synchronizing both magnetic fields, a stable phase information is gained. In this research, it is shown that the amplitude of the dominant mixing component is proportional to the amount of superparamagnetic beads inside a sample. Additionally, it is shown that the phase does not show this behaviour. Excitation frequency scans of different bead types were performed, showing different phases, without correlation to their diverse amplitudes. Two commercially available beads were selected and a determination of their amount in a mixture is performed as a demonstration for multiplex measurements.

摘要

相似文献

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[5]
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[6]
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[7]
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[9]
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[10]
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本文引用的文献

[1]
3D Printed Modular Immunofiltration Columns for Frequency Mixing-Based Multiplex Magnetic Immunodetection.

Sensors (Basel). 2019-1-3

[2]
Detecting Biothreat Agents: From Current Diagnostics to Developing Sensor Technologies.

ACS Sens. 2018-9-11

[3]
Biosensing Using Magnetic Particle Detection Techniques.

Sensors (Basel). 2017-10-10

[4]
Multi-color magnetic nanoparticle imaging using magnetorelaxometry.

Phys Med Biol. 2017-4-21

[5]
Portable, one-step, and rapid GMR biosensor platform with smartphone interface.

Biosens Bioelectron. 2016-4-19

[6]
Magnetic particle characterization-magnetophoretic mobility and particle size.

Cytometry A. 2016-6

[7]
Simple and portable magnetic immunoassay for rapid detection and sensitive quantification of plant viruses.

Appl Environ Microbiol. 2015-5-1

[8]
Recent advances in recognition elements of food and environmental biosensors: a review.

Biosens Bioelectron. 2010-7-17

[9]
Chip-NMR biosensor for detection and molecular analysis of cells.

Nat Med. 2008-8

[10]
CRP determination based on a novel magnetic biosensor.

Biosens Bioelectron. 2007-1-15

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