Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, Shandong 264003, China.
College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China.
FEMS Microbiol Lett. 2019 Jul 1;366(13). doi: 10.1093/femsle/fnz153.
Butyrate, a key metabolite fermented by gut microbiota mainly from undigested carbohydrates such as dietary fibers is widely used as feed additive. However, mechanisms of its contributions in maintaining host health are relatively poorly revealed. The aim of this study was to investigate how butyrate impacts gut microbiota and immunity response in high-fat diet-fed mice. Gut microbial analysis exhibited that butyrate intervention increased short-chain fatty acids (SCFAs)-producing bacteria and decreased pathogenic bacteria, such as endotoxin-secreting bacteria. Our result also demonstrated that butyrate intervention enhanced fecal SCFAs concentrations, and inhibited endotoxin levels in feces and serum. Correlation analysis indicated positive relation between endotoxin level and Desulfovibrionaceae abundance. Furthermore, butyrate intervention inhibited expressions of IL-1β, IL-6 and MCP1/CCL2 in liver, as well as TLR4 in adipose tissue. Apart from inhibiting expressions of proinflammatory cytokines, butyrate exerted anti-inflammation effect through selectively modulating gut microbiota, such as increasing SCFAs-producing bacteria and decreasing endotoxin-secreting bacteria, as well as via regulating levels of microbiota-dependent metabolites and components, such as SCFAs and endotoxin.
丁酸盐是肠道微生物发酵的一种关键代谢产物，主要来源于未消化的碳水化合物，如膳食纤维，被广泛用作饲料添加剂。然而，其在维持宿主健康方面的作用机制尚不清楚。本研究旨在探讨丁酸盐如何影响高脂肪饮食喂养小鼠的肠道微生物群和免疫反应。肠道微生物分析表明，丁酸盐干预增加了短链脂肪酸（SCFAs）产生菌，减少了产内毒素的细菌，如内毒素分泌菌。我们的结果还表明，丁酸盐干预增强了粪便中 SCFAs 的浓度，并抑制了粪便和血清中的内毒素水平。相关性分析表明，内毒素水平与脱硫弧菌丰度呈正相关。此外，丁酸盐干预抑制了肝脏中 IL-1β、IL-6 和 MCP1/CCL2 的表达，以及脂肪组织中 TLR4 的表达。除了抑制促炎细胞因子的表达外，丁酸盐还通过选择性调节肠道微生物群发挥抗炎作用，如增加 SCFAs 产生菌和减少产内毒素菌，以及通过调节微生物依赖性代谢物和成分的水平，如 SCFAs 和内毒素。
