Bhatnagar Sharad, Kobori Toshiro, Ganesh Deepak, Ogawa Kazuyoshi, Aoyagi Hideki
Life Science and Bioengineering, Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1, Tennodai, Tsukuba 305-8572, Ibaraki, Japan.
Food Research Institute, National Agriculture and Food Research Organization, 2-1-2, Kannondai, Tsukuba 305-8642, Ibaraki, Japan.
Nanomaterials (Basel). 2019 Jul 21;9(7):1042. doi: 10.3390/nano9071042.
In recent years, green syntheses have been researched comprehensively to develop inexpensive and eco-friendly approaches for the generation of nanoparticles. In this context, plant and microbial sources are being examined to discover potential reducing agents. This study aims to utilize an extracellular pigment produced by as a prospective reducing agent to synthesize silver nanoparticles (AgNPs). Biosynthesized AgNPs were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), electron probe micro analyser (EPMA), and zeta potential. The pigment functional groups involved in the generation of AgNPs were investigated using Fourier transform infrared spectroscopy. TEM images showed that the generated nanoparticles were spherical, hexagonal, rod-shaped, and triangular-shaped with a particle size distribution from 4 to 41 nm and exhibited a surface plasmon resonance at around 410 nm. DLS and zeta potential studies revealed that the particles were polydispersed and stable (-24.8 mV). EPMA confirmed the presence of elemental silver in the samples. Biosynthesized AgNPs exhibited minimum inhibitory concentrations of 32 and 4 μg/mL against and , respectively. Further, cytotoxicity of the AgNPs was investigated against human cervical cancer (HeLa), human liver cancer (HepG2), and human embryonic kidney (HEK-293) cell lines using 5-fluorouracil as a positive control. A significant activity was recorded against HepG2 cell line with a half-maximal inhibitory concentration of 11.1 μg/mL.
近年来，人们对绿色合成进行了全面研究，以开发用于生成纳米颗粒的廉价且环保的方法。在此背景下，正在研究植物和微生物来源以发现潜在的还原剂。本研究旨在利用由[具体微生物名称未给出]产生的一种细胞外色素作为一种潜在的还原剂来合成银纳米颗粒（AgNPs）。通过透射电子显微镜（TEM）、动态光散射（DLS）、电子探针微分析仪（EPMA）和zeta电位对生物合成的AgNPs进行了表征。使用傅里叶变换红外光谱研究了参与AgNPs生成的色素官能团。TEM图像显示，生成的纳米颗粒为球形、六边形、棒状和三角形，粒径分布为4至41 nm，并在约410 nm处表现出表面等离子体共振。DLS和zeta电位研究表明，颗粒是多分散的且稳定的（-24.8 mV）。EPMA证实了样品中存在元素银。生物合成的AgNPs对[具体细菌名称未给出]和[具体细菌名称未给出]的最低抑菌浓度分别为32和4 μg/mL。此外，以5-氟尿嘧啶作为阳性对照，研究了AgNPs对人宫颈癌（HeLa）、人肝癌（HepG2）和人胚肾（HEK-293）细胞系的细胞毒性。对HepG2细胞系记录到显著活性，半数最大抑制浓度为11.1 μg/mL。
