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通过单克隆抗体检测成骨过程中的fos蛋白。

Detection of fos protein during osteogenesis by monoclonal antibodies.

作者信息

De Togni P, Niman H, Raymond V, Sawchenko P, Verma I M

机构信息

Salk Institute, San Diego, California 92138.

出版信息

Mol Cell Biol. 1988 May;8(5):2251-6. doi: 10.1128/mcb.8.5.2251-2256.1988.

DOI:10.1128/mcb.8.5.2251-2256.1988
PMID:3133556
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC363412/
Abstract

We have generated monoclonal antibodies by using a synthetic peptide corresponding to amino acid positions 4 to 17 of the human fos protein. The antibodies detected both v- and c-fos proteins by immunoprecipitation, immunoblotting, and indirect immunofluorescence. The monoclonal antibodies not only identified the fos protein complex with the cellular 39-kilodalton protein, but also recognized the modified forms of the mouse, rat, and human fos proteins. In day-17 rat embryos, nuclear-staining fos protein could be identified in the cartilage by immunohistochemical staining.

摘要

我们通过使用与人Fos蛋白第4至17位氨基酸相对应的合成肽产生了单克隆抗体。这些抗体通过免疫沉淀、免疫印迹和间接免疫荧光检测到了v-Fos和c-Fos蛋白。这些单克隆抗体不仅鉴定出了与细胞39千道尔顿蛋白形成的Fos蛋白复合物，还识别出了小鼠、大鼠和人类Fos蛋白的修饰形式。在第17天的大鼠胚胎中，通过免疫组织化学染色可在软骨中鉴定出核染色的Fos蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e150/363412/b58ec136eae7/molcellb00065-0405-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e150/363412/68c7ba6676a5/molcellb00065-0402-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e150/363412/4c314e64dbed/molcellb00065-0403-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e150/363412/bc8e3f03ae8d/molcellb00065-0404-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e150/363412/b58ec136eae7/molcellb00065-0405-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e150/363412/68c7ba6676a5/molcellb00065-0402-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e150/363412/4c314e64dbed/molcellb00065-0403-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e150/363412/bc8e3f03ae8d/molcellb00065-0404-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e150/363412/b58ec136eae7/molcellb00065-0405-a.jpg

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本文引用的文献

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Molecular dissection of Rauscher virus gp70 by using monoclonal antibodies: localization of acquired sequences of related envelope gene recombinants.利用单克隆抗体对劳斯氏病毒糖蛋白70进行分子剖析：相关包膜基因重组体获得序列的定位
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