Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Villaviciosa, 33300 Asturias, Spain.
Diet, Microbiota and Health Group, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), 33011 Oviedo, Spain.
Nutrients. 2019 Jul 31;11(8):1765. doi: 10.3390/nu11081765.
The fecal microbiota plays an important role in human health, and alterations in the microbiota-host interaction seem to be involved in the ageing process. Therefore, it is of interest to develop strategies for promoting a balanced microbiota in old age in order to prevent the physiological and immune decline associated with age. However, the specific microbiota changes in the transition from adulthood to senescence are not yet well understood. Here we assessed the levels of some intestinal microorganisms and short chain fatty acids (SCFAs) across different age-groups. In total, 153 adults from four age groups (<50, 50-65, 66-80, and >80 years-old) were recruited; the levels of different bacterial groups in fecal samples were determined by quantitative polymerase chain reaction (qPCR), and those of SCFA by gas chromatography. Dietary information was collected by using a Food Frequency Questionnaire. The presence of the , , group, and cluster XIVa decreased with age up to 66-80 years of age, with differences reaching statistical significance for the latter group. Interestingly, the levels of some of these microorganisms recovered in the very old age group (>80 years), with these older individuals presenting significantly higher counts of and group than adults and the younger elderly. In addition, ageing was associated with a progressively and statistically significant reduction in the fecal concentrations of SCFAs. Dietary intakes also showed some statistically significant differences among the groups for some macro- and micronutrients. Moreover, associations of some microorganisms with age and macronutrients were also evidenced. Considering the role that fecal microbiota alterations may have in terms of impairing homeostasis and resilience, our results underline the importance of understanding the ageing and immunosenescence processes by including the microbiota perspective.
肠道微生物群在人类健康中发挥着重要作用，微生物群与宿主的相互作用的改变似乎与衰老过程有关。因此，开发促进老年平衡微生物群的策略以预防与年龄相关的生理和免疫下降是很有意义的。然而，从成年到衰老过渡过程中特定的微生物群变化尚不清楚。在这里，我们评估了不同年龄组的一些肠道微生物和短链脂肪酸 (SCFA) 的水平。总共招募了 153 名来自四个年龄组 (<50、50-65、66-80 和 >80 岁) 的成年人；通过定量聚合酶链反应 (qPCR) 测定粪便样本中不同细菌群的水平，通过气相色谱法测定 SCFA 的水平。通过使用食物频率问卷收集饮食信息。,, 群和 14a 簇的存在随着年龄增长至 66-80 岁而下降，对于后者组差异达到统计学意义。有趣的是，其中一些微生物的水平在非常老年组（>80 岁）中恢复，这些年龄较大的个体的 和 群计数明显高于成年人和年轻的老年人。此外，衰老与粪便 SCFA 浓度逐渐且具有统计学意义的减少有关。饮食摄入量在某些宏量和微量营养素方面在各组之间也显示出一些具有统计学意义的差异。此外，还证明了一些微生物与年龄和宏量营养素之间的关联。考虑到粪便微生物群改变可能在破坏体内平衡和恢复力方面的作用，我们的结果强调了通过包括微生物组的观点来理解衰老和免疫衰老过程的重要性。
