Semba K, Nishizawa M, Satoh H, Fukushige S, Yoshida M C, Sasaki M, Matsubara K, Yamamoto T, Toyoshima K
Department of Oncology, University of Tokyo.
Jpn J Cancer Res. 1988 Jun;79(6):710-7. doi: 10.1111/j.1349-7006.1988.tb02227.x.
We molecularly characterized the second gene, c-yes-2, of two copies of yes-related genes which we previously found to contain in the human genome. First, nucleotide sequence analysis revealed that the c-yes-2 gene is a pseudogene of the c-yes-1 gene. Second, by using two independent methods, hybridization of both DNAs from sorted chromosomes and metaphase spreads with c-yes-2 DNA, we assigned the c-yes-2 gene to chromosome 22q11.2. This chromosomal localization is consistent with that given in our previous report. The failure of proper mapping in our experiment might have been caused by instability of hybrid cell clones.
我们对之前发现的人类基因组中两个与yes相关基因拷贝中的第二个基因c-yes-2进行了分子特征分析。首先，核苷酸序列分析表明c-yes-2基因是c-yes-1基因的假基因。其次，通过两种独立方法，即对分选染色体和中期染色体铺展的DNA与c-yes-2 DNA进行杂交，我们将c-yes-2基因定位于22q11.2染色体。这一染色体定位与我们之前报告中的定位一致。我们实验中正确定位失败可能是由杂交细胞克隆的不稳定性导致的。
