Alrumaihi Faris
Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraidah, Saudi Arabia.
Int J Health Sci (Qassim). 2019 Sep-Oct;13(5):31-36.
commonly known as Bilsaan is used extensively in traditional medicine in Saudi Arabia to treat various types of illness. The establishment of safe dose of is crucial while considering health-promoting benefits as exposure to excess amount may cause undesirable effects. The aim of the present study was to evaluate the acute oral toxicity of ethanolic (Eth) and methanolic (Meth) extracts from the bark of Bilsaan (Bil) in mice.
The acute oral toxicity study of Eth-Bil and Meth-Bil extracts was carried out by the administration of 10, 100, and 1000 mg/kg body weight to mice in the 1 phase. According to the observation of the 1 phase, the Eth-Bil extract was given 50, 100, 200, and 400 while Meth-Bil was administered 200, 400, 800, and 1600 mg/kg body weight.
The LD of the (Bilsaan) methanol and ethanol extracts in mice was determined to be 31.62 mg/kg body weight for both of the extracts as calculated by Lorke's method. The gross observation of systemic organs demonstrated 10-90% increase in the weight of organs of animals treated with increasing concentration of extracts in comparison to control. Similarly, the hematological analyses also showed abnormality and reduction in total leukocytes count exposed with the higher concentration of extracts.
The results indicate that the oral administration of Eth-Bil and Meth-Bil extracts produce a significant toxic effect in mice but support in the establishment of safe dose range, as the intake of high doses of (Bilsaan) extracts may exhibit mild organ toxicity.
在沙特阿拉伯传统医学中广泛使用的比尔萨安（Bilsaan）常用于治疗各类疾病。在考虑其促进健康的益处时，确定其安全剂量至关重要，因为接触过量可能会产生不良影响。本研究的目的是评估比尔萨安树皮的乙醇提取物（Eth）和甲醇提取物（Meth）对小鼠的急性经口毒性。
在第1阶段，通过给小鼠灌胃10、100和1000mg/kg体重进行Eth-Bil和Meth-Bil提取物的急性经口毒性研究。根据第1阶段的观察结果，Eth-Bil提取物给予50、100、200和400mg/kg体重，而Meth-Bil给予200、400、800和1600mg/kg体重。
根据洛尔凯（Lorke）法计算，两种提取物（比尔萨安）的甲醇和乙醇提取物对小鼠的半数致死量（LD）均为31.62mg/kg体重。对全身器官的大体观察表明，与对照组相比，随着提取物浓度增加，接受治疗的动物器官重量增加了10%-90%。同样，血液学分析也显示，接触高浓度提取物会出现异常并导致白细胞总数减少。
结果表明，口服Eth-Bil和Meth-Bil提取物对小鼠产生显著毒性作用，但有助于确定安全剂量范围，因为摄入高剂量的（比尔萨安）提取物可能会表现出轻度器官毒性。
