Soares Mariana Quirino Silveira, Van Dessel Jeroen, Jacobs Reinhilde, Yaedú Renato Yassutaka Faria, Sant'Ana Eduardo, da Silva Corrêa Danilo, Madeira Maria Fernanda Conceição, Duarte Marco Antônio Húngaro, Rubira-Bullen Izabel Regina Fischer
Department of Surgery, Stomatology, Pathology and Radiology, Bauru School of Dentistry, University of São Paulo, Bauru, 17012-901, Brazil.
OMFS-IMPATH research group, Department of Imaging and Pathology, Faculty of Medicine, KU Leuven and Oral and Maxillofacial Surgery, University Hospitals Leuven, Leuven, Belgium.
Int J Implant Dent. 2019 Sep 10;5(1):32. doi: 10.1186/s40729-019-0187-1.
Bovine bone grafts have been widely used in dentistry for guided tissue regeneration and can support new bone formation in direct contact with the graft. The aim of this study was to compare the morphometric and bone density changes after using two different bovine bone graft blocks in segmental osseous defects in the mandible of rabbits following different postoperative periods.
Critical size segmental defects were surgically created bilaterally in the jaw of 18 rabbits. The defects were filled with either deproteinized bovine bone mineral with 10% collagen (DBBM-C; BioOss Collagen®), lyophilized bovine medullary bone (LBMB; Orthogen®), or left untreated according to a split-mouth design. Animals were sacrificed after 3 or 6 months of healing. The hemimandibles were scanned ex vivo using a high-resolution (19 μm) microcomputed tomography. Morphometric and bone density parameters were calculated in the region of the defect using CT-Analyser (Bruker). Initial graft blocks were used as baseline.
DBBM-C presented a denser microarchitecture, in comparison to LBMB at baseline. DBBM-C and LBMB grafted regions showed a similar progressive remodeling, with a significant decrease in structure complexity and maintenance of bone volume fraction during the postoperative follow-up periods. Both graft materials showed an enhanced bone replacement and more complex structure compared to untreated defects. The apparent fusion between the graft and host bone was observed only in the defects filled with LBMB.
LBMB grafts showed a similar behavior as DBBM-C regarding structural remodeling. In LBMB samples, apparent integration between the host bone and the graft was present.
牛骨移植材料已在牙科领域广泛用于引导组织再生，并且能够支持与移植材料直接接触部位的新骨形成。本研究的目的是比较在兔下颌骨节段性骨缺损中使用两种不同的牛骨移植块后，在不同术后时期的形态学和骨密度变化。
对18只兔的双侧颌骨进行手术制造临界尺寸节段性缺损。根据双侧对照设计，缺损分别填充含10%胶原蛋白的脱蛋白牛骨矿物质（DBBM-C；BioOss Collagen®）、冻干牛髓质骨（LBMB；Orthogen®），或不进行处理。在愈合3或6个月后处死动物。使用高分辨率（19μm）微型计算机断层扫描对离体半侧下颌骨进行扫描。使用CT-Analyser（布鲁克公司）在缺损区域计算形态学和骨密度参数。将初始移植块用作基线。
与基线时的LBMB相比，DBBM-C呈现出更致密的微观结构。DBBM-C和LBMB移植区域显示出相似的渐进性重塑，在术后随访期间结构复杂性显著降低，骨体积分数保持不变。与未处理的缺损相比，两种移植材料均显示出增强的骨替代和更复杂的结构。仅在填充LBMB的缺损中观察到移植材料与宿主骨之间明显融合。
在结构重塑方面，LBMB移植材料表现出与DBBM-C相似的特性。在LBMB样本中，宿主骨与移植材料之间存在明显整合。
