Adusei Emmanuel B A, Adosraku Reimmel K, Oppong-Kyekyeku James, Amengor Cedric D K
Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Department of Pharmaceutical Chemistry, School of Pharmacy, University of Health and Allied Sciences, Ho, Ghana.
Int J Anal Chem. 2019 Aug 18;2019:4061927. doi: 10.1155/2019/4061927. eCollection 2019.
There has been an increasing interest in the search for colour indicators of natural origin for titrimetric analysis. This is due to some challenges associated with the currently used synthetic ones. This study evaluates and validates the acid-base indicator property of plumbagin isolated from Linn. Plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone) was isolated from the roots of Linn using silica gel chromatography and characterized using spectroscopic methods in comparison with those reported in the literature. Its acid-base indicator property was evaluated alongside phenolphthalein and methyl orange, after it was found to exhibit a sharp change in colour at various pH ranges. The plumbagin indicator was successfully used to assay ibuprofen powder and tablets (400 mg) using the British Pharmacopoeia (2013) method. Data obtained were analyzed statistically by Student's -test and one-way ANOVA in GraphPad Prism (version 5.01, 2010). Analysis of the use of the plumbagin indicator in acid-base titrations between strong acids and strong bases and between weak acids and strong bases has been evaluated and validated according to the ICH guidelines. Plumbagin use in ibuprofen powder and tablets has also been verified. Plumbagin has been validated for use as an indicator suitable for different acid-base titrations and the analysis of ibuprofen.
对于寻找用于滴定分析的天然来源颜色指示剂的兴趣与日俱增。这是由于目前使用的合成指示剂存在一些挑战。本研究评估并验证了从白花丹属植物中分离出的白花丹素的酸碱指示剂性质。白花丹素（5-羟基-2-甲基-1,4-萘醌）通过硅胶柱色谱法从白花丹属植物的根中分离得到，并与文献报道的方法进行比较，采用光谱法进行表征。在发现其在不同pH范围内颜色有明显变化后，将其酸碱指示剂性质与酚酞和甲基橙一起进行了评估。采用英国药典（2013年版）方法，白花丹素指示剂成功用于布洛芬粉末和片剂（400毫克）的含量测定。在GraphPad Prism（版本5.01，2010年）中，使用学生t检验和单因素方差分析对所得数据进行统计学分析。根据国际人用药品注册技术协调会（ICH）指南，对白花丹素指示剂在强酸与强碱以及弱酸与强碱之间的酸碱滴定中的应用进行了评估和验证。白花丹素在布洛芬粉末和片剂中的应用也得到了验证。白花丹素已被验证可作为适用于不同酸碱滴定以及布洛芬分析的指示剂。
