Szentágothai Research Centre, Virological Research Group Pécs Hungary, University of Pécs, 7624 Pécs, Hungary.
Institute of Biology, Faculty of Sciences, University of Pécs, 7622 Pécs, Hungary.
Viruses. 2019 Sep 21;11(10):887. doi: 10.3390/v11100887.
In the past ten years, several novel hantaviruses were discovered in shrews, moles, and bats, suggesting the dispersal of hantaviruses in many animal taxa other than rodents during their evolution. Interestingly, the coevolutionary analyses of most recent studies have raised the possibility that nonrodents may have served as the primordial mammalian host and harboured the ancestors of rodent-borne hantaviruses as well. The aim of our study was to investigate the presence of hantaviruses in bat lung tissue homogenates originally collected for taxonomic purposes in Malaysia in 2015. Hantavirus-specific nested RT-PCR screening of 116 samples targeting the L segment of the virus has revealed the positivity of two lung tissue homogenates originating from two individuals, a female and a male of the bat species collected at the same site and sampling occasion. Nanopore sequencing of hantavirus positive samples resulted in partial genomic data from S, M, and L genome segments. The obtained results indicate molecular evidence for hantaviruses in the bat species. Sequence analysis of the PCR amplicon and partial genome segments suggests that the identified virus may represent a novel species in the genus within the Hantaviridae family. Our results provide additional genomic data to help extend our knowledge about the evolution of these viruses.
在过去的十年中，人们在鼩鼱、鼹鼠和蝙蝠中发现了几种新型汉坦病毒，这表明在进化过程中，汉坦病毒在许多非啮齿动物类群中传播。有趣的是，最近的研究中的共进化分析提出了一种可能性，即非啮齿动物可能曾经作为原始哺乳动物宿主，并携带了啮齿动物传播的汉坦病毒的祖先。我们的研究目的是调查 2015 年在马来西亚为分类学目的而收集的蝙蝠肺组织匀浆中是否存在汉坦病毒。使用针对病毒 L 片段的嵌套 RT-PCR 对 116 个样本进行汉坦病毒特异性筛选，结果显示来自同一地点和采样时间采集的两只蝙蝠个体的两个肺组织匀浆呈阳性。对汉坦病毒阳性样本进行纳米孔测序得到了 S、M 和 L 基因组片段的部分基因组数据。获得的结果表明，在蝙蝠物种中存在汉坦病毒的分子证据。PCR 扩增子和部分基因组片段的序列分析表明，所鉴定的病毒可能代表汉坦病毒科属内的一个新种。我们的研究结果提供了额外的基因组数据，有助于扩展我们对这些病毒进化的认识。
