Thai Nguyen University of Sciences, Thai Nguyen City, Thai Nguyen province 250000, Vietnam.
Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do 16227, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Jan;70(1):656-661. doi: 10.1099/ijsem.0.003811.
A rod-shaped, Gram-stain-positive bacterial strain, MS74, was isolated from soil beside Itaewon road, Seoul, Republic of Korea. The strain could grow well on R2A, nutrient agar and tryptone soya agar, but not in LB agar. MS74 tolerated 3.0 % NaCl (w/v), pH 9.0 and a temperature range from 10 to 35 °C (optimal temperature, 28 °C). From the comparison of 16S rRNA gene sequence, the strain is most closely related to CCUG 53270 CFH S0170, JCM 16352 and YN2 with similarity percentages of 96.6 %, 96.4 %, 95.9 % and 95.8 % respectively. Major polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), and phosphatidylethanolamine (PE). The major menaquinone was MK-7. The fatty acids profile mainly consisted of C anteiso, C iso, C iso, and C. The DNA G+C content of the isolated strain determined from the whole-genome sequence was 51 mol%. MS74 had an average nucleotide identity (ANI) value of 73.9 % and a digital DNA-DNA hybridization (dDDH) value of 20.6 % with most closely related strain, CCUG 53270. On the basis of phenotypic, chemotypic and genotypic evidence, the isolate was identified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MS74 (=KACC 19385 =DSM 105496).
一株杆状、革兰氏阳性的细菌菌株 MS74 是从韩国首尔梨泰院路旁边的土壤中分离出来的。该菌株可以在 R2A、营养琼脂和胰蛋白胨大豆琼脂上良好生长,但不能在 LB 琼脂上生长。MS74 可以耐受 3.0%NaCl(w/v)、pH9.0 和 10-35°C 的温度范围(最适温度为 28°C)。通过 16S rRNA 基因序列的比较,该菌株与 CCUG 53270 CFH S0170、JCM 16352 和 YN2 最为密切相关,相似性百分比分别为 96.6%、96.4%、95.9%和 95.8%。主要极性脂为双磷脂酰甘油(DPG)、磷脂酰甘油(PG)和磷脂酰乙醇胺(PE)。主要的menaquinone 是 MK-7。脂肪酸图谱主要由 C anteiso、C iso、C iso 和 C 组成。从全基因组序列确定的分离菌株的 DNA G+C 含量为 51mol%。MS74 与最密切相关的菌株 CCUG 53270 的平均核苷酸同一性(ANI)值为 73.9%,数字 DNA-DNA 杂交(dDDH)值为 20.6%。基于表型、化学型和基因型证据,该分离株被鉴定为代表一个新的属种,因此提议使用 sp. nov. 作为新种名。模式菌株为 MS74(=KACC 19385=DSM 105496)。