Priority Research Centre for Healthy Lungs, The University of Newcastle, Newcastle, NSW, Australia.
Viruses, Infection & Immunity, Vaccines & Asthma (VIVA), Hunter Medical Research Institute, Newcastle, NSW, Australia.
Respir Res. 2019 Nov 20;20(1):259. doi: 10.1186/s12931-019-1225-5.
Respiratory virus-induced asthma exacerbations occur frequently during pregnancy and are associated with adverse outcomes for mother and child. Primary nasal epithelial cells (pNECs) provide a useful method to study immune responses in pregnancy. pNECs were obtained by nasal brushings from pregnant and non-pregnant women with and without asthma. pNECS were infected in vitro with major group Rhinovirus 43 (RV43) and seasonal influenza (H3N2). Following infection, pNECs showed measurable quantities of interferon (IFN)-λ, IL-1β, IL-8, IP-10 and MIP1-α. pNECs provide a safe and effective method for studying respiratory epithelial cell responses during pregnancy.
呼吸道病毒引起的哮喘恶化在怀孕期间经常发生，并与母婴的不良结局相关。主要的鼻上皮细胞（pNEC）为研究妊娠期间的免疫反应提供了一种有用的方法。pNEC 是通过鼻刷从有和没有哮喘的孕妇和非孕妇中获得的。pNEC 在体外感染主要组鼻病毒 43（RV43）和季节性流感（H3N2）。感染后，pNEC 显示出可测量数量的干扰素（IFN）-λ、IL-1β、IL-8、IP-10 和 MIP1-α。pNEC 为研究妊娠期间呼吸道上皮细胞反应提供了一种安全有效的方法。
