Department of Oncology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.
State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200032, China.
J Nanobiotechnology. 2019 Nov 25;17(1):116. doi: 10.1186/s12951-019-0548-1.
Tumor cells with heterogeneity and diversity can express different markers. At present, positive separation of circulating tumor cells (CTC) taking EpCAM as the marker was used in most cases which could be one-sided, while this study successfully prepared four antibody-modified magnetic immunoliposomes (MIL) by using the self-assembled liposome with antibody derivatives. This study aims to explore the separation efficiency and clinical detection feasibility of single or combined use of MIL with multi-tumor markers on different tumors. Captured CTC were stained with CK-FITC, CD45-PE and DAPI, and fluorescence microscope was used for the observation, analysis and calculation. The result indicated that the CTC number positive rate in blood samples of four different magnetic balls on the same patient could be up to 87.5% in 32 patients with 14 different kinds tumors. While the effect of directly mixed separation by four kinds of magnetic balls was not satisfying. It suggested that the MIL of multi-tumor markers could be a powerful tool for CTC separation in application of tumor screening and prognosis.
具有异质性和多样性的肿瘤细胞可以表达不同的标志物。目前，大多数情况下采用以 EpCAM 为标志物的阳性分离循环肿瘤细胞（CTC），可能具有片面性，而本研究通过使用抗体衍生物自组装的脂质体，成功制备了四种抗体修饰的磁性免疫脂质体（MIL）。本研究旨在探索 MIL 与多肿瘤标志物联合使用对不同肿瘤的分离效率和临床检测可行性。用 CK-FITC、CD45-PE 和 DAPI 染色捕获的 CTC，并用荧光显微镜进行观察、分析和计算。结果表明，在 32 名患有 14 种不同肿瘤的患者的血液样本中，四种不同磁珠上的 CTC 阳性率最高可达 87.5%。而四种磁珠直接混合分离的效果并不理想。这表明多肿瘤标志物的 MIL 可能是肿瘤筛查和预后应用中 CTC 分离的有力工具。
