载有 ZnO 纳米粒子和蜂胶提取物的细菌纤维素薄膜：协同抗菌作用。
Bacterial cellulose films with ZnO nanoparticles and propolis extracts: Synergistic antimicrobial effect.
机构信息
University POLITEHNICA of Bucharest, Faculty of Applied Chemistry and Materials Science, Gh. Polizu Street 1-7, postal code 011061, Bucharest, Romania.
Research Center for Instrumental Analysis - SCIENT, Petre Ispirescu Street 1, Tâncăbeşti, postal code 077167, Ilfov, Romania.
出版信息
Sci Rep. 2019 Nov 27;9(1):17687. doi: 10.1038/s41598-019-54118-w.
This study aimed to obtain possible materials for future antimicrobial food packaging applications based on biodegradable bacterial cellulose (BC). BC is a fermentation product obtained by Gluconacetobacter xylinum using food or agricultural wastes as substrate. In this work we investigated the synergistic effect of zinc oxide nanoparticles (ZnO NPs) and propolis extracts deposited on BC. ZnO NPs were generated in the presence of ultrasounds directly on the surface of BC films. The BC-ZnO composites were further impregnated with ethanolic propolis extracts (EEP) with different concentrations.The composition of raw propolis and EEP were previously determined by gas-chromatography mass-spectrometry (GC-MS), while the antioxidant activity was evaluated by TEAC (Trolox equivalent antioxidant capacity). The analysis methods performed on BC-ZnO composites such as scanning electron microscopy (SEM), thermo-gravimetrically analysis (TGA), and energy-dispersive X-ray spectroscopy (EDX) proved that ZnO NPs were formed and embedded in the whole structure of BC films. The BC-ZnO-propolis films were characterized by SEM and X-ray photon spectroscopy (XPS) in order to investigate the surface modifications. The antimicrobial synergistic effect of the BC-ZnO-propolis films were evaluated against Escherichia coli, Bacillus subtilis, and Candida albicans. The experimental results revealed that BC-ZnO had no influence on Gram-negative and eukaryotic cells.
本研究旨在基于可生物降解的细菌纤维素（BC）获得未来抗菌食品包装应用的可能材料。BC 是一种发酵产物，由木醋杆菌使用食品或农业废物作为基质获得。在这项工作中，我们研究了氧化锌纳米粒子（ZnO NPs）和蜂胶提取物在 BC 上沉积的协同效应。在 BC 薄膜表面直接存在超声的情况下生成 ZnO NPs。进一步将 BC-ZnO 复合材料用不同浓度的乙醇蜂胶提取物（EEP）浸渍。通过气相色谱-质谱联用（GC-MS）预先确定了原始蜂胶和 EEP 的组成，而通过 TEAC（Trolox 等效抗氧化能力）评估了抗氧化活性。对 BC-ZnO 复合材料进行的分析方法，如扫描电子显微镜（SEM）、热重分析（TGA）和能量色散 X 射线光谱（EDX）证明，ZnO NPs 已形成并嵌入 BC 薄膜的整个结构中。通过 SEM 和 X 射线光电子能谱（XPS）对 BC-ZnO-蜂胶薄膜进行了表征，以研究表面改性。评估了 BC-ZnO-蜂胶薄膜对大肠杆菌、枯草芽孢杆菌和白色念珠菌的抗菌协同作用。实验结果表明，BC-ZnO 对革兰氏阴性和真核细胞没有影响。
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