Mahmoudi-Nezhad Mahsa, Farhangi Mahdieh Abbasalizad, Kahroba Houman
Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Attar-Neishabouri Ave, Golgasht St, Tabriz, 5165665931, Iran.
J Transl Med. 2020 Jan 9;18(1):16. doi: 10.1186/s12967-020-02208-z.
Epidemiologic studies show that cocaine- and amphetamine-regulated transcript prepropeptide (CARTPT) gene polymorphism modifies diet-obesity relationships. However, the interaction between CARTPT gene polymorphism and diet quality indices have not been investigated yet. The current study was aimed to evaluate the interaction between major dietary indices including Diet Quality Index-International (DQI-I) and Healthy Eating Index (HEI)-2015 and CARTPT gene rs2239670 variants among apparently healthy obese Iranians.
This cross-sectional study was carried out by employing 288 apparently healthy obese adults aged 20-50 years with a BMI of 30-40 kg/m. Diet quality was evaluated by Diet Quality Index-International (DQI-I) and Healthy Eating Index-2015 (HEI-2015) using a 132-items semi-quantitative validated food frequency questionnaire. The CARTPT gene rs2239670 polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Blood concentrations of glycemic markers, lipid profile, α-melanocyte stimulating hormone (MSH) and agouti-related peptide (AgRP) were also measured. ANCOVA multivariate interaction model was used to analyze gene-diet interactions.
The significant interactions were identified between CARTPT gene polymorphism and HEI, affecting BMR (P = 0.003), serum glucose (P = 0.009) and high density lipoprotein cholesterol HDL concentrations (P = 0.03) after adjusting for the effects of sex and age. Also we found gene-diet interaction between CARTPT genotypes and DQI-I in terms of fat mass (FM; P = 0.02), waist circumference (WC; P < 0.001), body mass index (BMI; P < 0.001), basal metabolic rate (BMR, P < 0.001), serum fasting glucose (P < 0.01) and AgRP (P = 0.05) in individuals even after adjusting for potential confounders.
Current study showed the effects of interaction between CARTPT genotype with adherence to HEI and DQI-I scores on obesity-related anthropometric and metabolic risk-factors.
流行病学研究表明，可卡因和苯丙胺调节转录前体肽（CARTPT）基因多态性会改变饮食与肥胖之间的关系。然而，CARTPT基因多态性与饮食质量指数之间的相互作用尚未得到研究。本研究旨在评估主要饮食指数，包括国际饮食质量指数（DQI-I）和2015年健康饮食指数（HEI）与明显健康的肥胖伊朗人CARTPT基因rs2239670变体之间的相互作用。
本横断面研究采用288名年龄在20至50岁之间、BMI为30至40kg/m²的明显健康的肥胖成年人。使用一份经过验证的132项半定量食物频率问卷，通过国际饮食质量指数（DQI-I）和2015年健康饮食指数（HEI-2015）评估饮食质量。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术对CARTPT基因rs2239670多态性进行基因分型。还测量了血糖标志物、血脂谱、α-黑素细胞刺激素（MSH）和刺鼠相关肽（AgRP）的血液浓度。使用协方差分析多元相互作用模型分析基因与饮食的相互作用。
在调整性别和年龄的影响后，发现CARTPT基因多态性与HEI之间存在显著相互作用，影响基础代谢率（P = 0.003）、血清葡萄糖（P = 0.009）和高密度脂蛋白胆固醇HDL浓度（P = 0.03）。此外，即使在调整潜在混杂因素后，我们发现CARTPT基因型与DQI-I之间在脂肪量（FM；P = 0.02）、腰围（WC；P < 0.001）、体重指数（BMI；P < 0.001）、基础代谢率（BMR，P < 0.001）、空腹血糖（P < 0.01）和AgRP（P = 0.05）方面存在基因-饮食相互作用。
当前研究表明，CARTPT基因型与对HEI和DQI-I评分的依从性之间的相互作用对肥胖相关的人体测量和代谢风险因素有影响。
