人乳头状瘤病毒在患有宫颈上皮内瘤变的有性生活史的女性唾液中的流行率。
Prevalence of human papillomavirus in the saliva of sexually active women with cervical intraepithelial neoplasias.
机构信息
Clínica Odontológica Universitaria Unidad Docente de Cirugía Bucal Hospital Morales Meseguer, 2 planta Avda. Marqués de los Vélez s/n 30008, Murcia, Spain
出版信息
Med Oral Patol Oral Cir Bucal. 2020 Mar 1;25(2):e195-e204. doi: 10.4317/medoral.23300.
BACKGROUND
The main objective of this study was to estimate the prevalence of human papillomavirus-DNA (HPV-DNA) in the saliva of sexually active women with HPV-related cervical intraepithelial neoplasias (CIN) and compare the findings with a healthy control group. The secondary objectives were: 1) to determine the concordance between genital and oral HPV types in sexually active women with HPV-related CIN; 2) to analyze whether sexual habits influence the presence of HPV-related CIN; 3) to determine whether sexual habits influence the presence of oral HPV.
MATERIAL AND METHODS
Saliva samples were collected from 100 sexually active women, 50 with HPV-related CIN and 50 healthy subjects presenting normal cytology. PCR assay was used to detect HPV-DNA.
RESULTS
The prevalence of oral HPV infection in saliva samples was 14% in women with HPV-related CIN, while in the healthy group it was 12%, without statistically significant difference (p=0.766). As for the concordance between genital and oral HPV types in women with HPV-related CIN, concordance was only observed for HPV-16, whereby among 22 women with genital HPV-16, only one (4.54%) also presented oral HPV-16. Regarding the possible influence of sexual habits on the presence of cervical pathology and presence of oral HPV, it was found that marital status, age at first intercourse, number of lifetime sexual partners, and condom use are related with the presence of cervical pathology (p<0.001; p=0.017; p=0.002; and p<0.001, respectively); condom use was also found to be related to the presence of oral HPV (p<0.001).
CONCLUSIONS
The prevalence of HPV-DNA in the saliva of sexually active women with HPV-related CIN is similar to healthy women. The concordance between genital and oral HPV types is low. Both the presence of cervical pathology and the presence of oral HPV are related to sexual habits. Wider sample size is required to confirm this results.
背景
本研究的主要目的是评估 HPV 相关宫颈上皮内瘤变（CIN）患者唾液中人乳头瘤病毒-DNA（HPV-DNA）的流行率，并与健康对照组进行比较。次要目的是：1）确定 HPV 相关 CIN 患者生殖道和口腔 HPV 类型的一致性；2）分析性行为习惯是否影响 HPV 相关 CIN 的发生；3）分析性行为习惯是否影响口腔 HPV 的发生。
材料与方法
收集 100 例活跃期有性生活的女性的唾液样本，其中 HPV 相关 CIN 患者 50 例，健康对照组 50 例，均行细胞学检查正常。采用 PCR 法检测 HPV-DNA。
结果
HPV 相关 CIN 患者唾液中 HPV 感染的流行率为 14%，而健康组为 12%，差异无统计学意义（p=0.766）。HPV 相关 CIN 患者生殖道和口腔 HPV 类型的一致性仅观察到 HPV-16，22 例生殖道 HPV-16 患者中，仅 1 例（4.54%）也存在口腔 HPV-16。关于性行为习惯对宫颈病变和口腔 HPV 发生的可能影响，发现婚姻状况、首次性行为年龄、性伴侣人数和避孕套使用与宫颈病变的发生有关（p<0.001；p=0.017；p=0.002；p<0.001）；避孕套使用与口腔 HPV 的发生也有关（p<0.001）。
结论
HPV 相关 CIN 患者唾液中 HPV-DNA 的流行率与健康女性相似。生殖道和口腔 HPV 类型的一致性较低。宫颈病变和口腔 HPV 的发生均与性行为习惯有关。需要更大的样本量来证实这些结果。
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