Department of Urology , The First Affiliated Hospital of Soochow University , NO. 188 Shizi Road , Suzhou 215006 , China.
Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Division of Life Science and State Key Laboratory of Molecular Neuroscience, Institute of Molecular Functional Materials, Department Chemical and Biological Engineering and Institute for Advanced Study , The Hong Kong University of Science and Technology , Clear Water Bay, Kowloon 999077 , Hong Kong , China.
ACS Appl Mater Interfaces. 2020 Feb 19;12(7):8040-8049. doi: 10.1021/acsami.9b22957. Epub 2020 Feb 4.
Iatrogenic ureteral injury is a dreaded complication of abdominal and pelvic surgeries, and thus, intraoperative identification of ureters is of paramount importance but lacks efficient methods and probes. Herein, we used near-infrared II (NIR-II, 1000-1700 nm) fluorescence imaging with advantages of higher spatial resolution, deeper tissue penetration, lower light scattering, and less tissue autofluorescence to identify ureters by aggregation-induced emission luminogen dots (AIE dots). The intraoperative ureteral injuries and common ureteral diseases can be visualized timely and precisely. Due to the longer emission wavelength and higher quantum yield of the AIE dots, it largely outperforms the commercial indocyanine green dye in brightness and penetration depth. It was the first time to realize the intraoperative identification of ureters in vivo using NIR-II imaging. Thus, our work provides a new platform for intraoperative monitoring during clinical operation.
医源性输尿管损伤是腹部和骨盆手术中一种可怕的并发症，因此，术中识别输尿管至关重要，但缺乏有效的方法和探针。在这里，我们使用近红外二区（NIR-II，1000-1700nm）荧光成像，具有更高的空间分辨率、更深的组织穿透性、更低的光散射和更少的组织自发荧光的优点，通过聚集诱导发射发光团点（AIE 点）来识别输尿管。术中输尿管损伤和常见输尿管疾病可以被及时、精确地可视化。由于 AIE 点的发射波长更长、量子产率更高，其亮度和穿透深度大大优于商业吲哚菁绿染料。这是首次使用近红外二区成像实现体内输尿管的术中识别。因此，我们的工作为临床手术中的术中监测提供了一个新的平台。
