Ten-Doménech Isabel, Ramos-Garcia Victoria, Piñeiro-Ramos José David, Gormaz María, Parra-Llorca Anna, Vento Máximo, Kuligowski Julia, Quintás Guillermo
Neonatal Research Unit, Health Research Institute La Fe, Avenida Fernando Abril Martorell 106, 46026 Valencia, Spain.
Division of Neonatology, University & Polytechnic Hospital La Fe, Avenida Fernando Abril Martorell 106, 46026 Valencia, Spain.
Metabolites. 2020 Jan 22;10(2):43. doi: 10.3390/metabo10020043.
Human milk (HM) is considered the gold standard for infant nutrition. HM contains macro- and micronutrients, as well as a range of bioactive compounds (hormones, growth factors, cell debris, etc.). The analysis of the complex and dynamic composition of HM has been a permanent challenge for researchers. The use of novel, cutting-edge techniques involving different metabolomics platforms has permitted to expand knowledge on the variable composition of HM. This review aims to present the state-of-the-art in untargeted metabolomic studies of HM, with emphasis on sampling, extraction and analysis steps. Workflows available from the literature have been critically revised and compared, including a comprehensive assessment of the achievable metabolome coverage. Based on the scientific evidence available, recommendations for future untargeted HM metabolomics studies are included.
母乳被视为婴儿营养的黄金标准。母乳含有大量营养素和微量营养素，以及一系列生物活性化合物（激素、生长因子、细胞碎片等）。对母乳复杂且动态的成分进行分析一直是研究人员面临的长期挑战。使用涉及不同代谢组学平台的新型前沿技术有助于扩展对母乳可变成分的认识。本综述旨在介绍母乳非靶向代谢组学研究的最新进展，重点关注采样、提取和分析步骤。对文献中可用的工作流程进行了严格修订和比较，包括对可实现的代谢组覆盖范围的全面评估。基于现有科学证据，还纳入了对未来母乳非靶向代谢组学研究的建议。
