Söderlund Robert, Skarin Hanna, Börjesson Stefan, Sannö Axel, Jernberg Therese, Aspán Anna, Ågren Erik O, Hansson Ingrid
Department of Microbiology, National Veterinary Institute, Uppsala, Sweden.
Division of Microbiology, Infection and Inflammation, Linköping University, Linköping, Sweden.
Infect Ecol Epidemiol. 2019 Dec 12;9(1):1701399. doi: 10.1080/20008686.2019.1701399. eCollection 2019.
: Wild birds pose a potential threat to animal and human health by spreading infectious diseases. In the present study, we studied the occurrence of bacterial zoonotic pathogens as well as enterobacteria with transferrable antimicrobial resistance genes among Swedish corvids. : Intestines from 66 jackdaws, crows, rooks and magpies from the vicinity of livestock farms at 14 locations in 7 counties were analysed by direct culture or PCR screening followed by culture. Isolates were investigated by whole-genome sequencing. : were detected in 82% and in 3% of the birds. ESBL-producing were found in one sample (2%) and carried . No with transferable carbapenem resistance were identified. No or O157:H7 were found, but PCR analysis for enterohaemorrhagic virulence genes revealed 35% positive samples for intimin, 9% for verotoxin 1 and 17% for verotoxin 2. isolates from corvids were compared to previously published isolates from Swedish sources by multi-locus sequence typing based on genome sequences. All corvid isolates formed a cluster, intermingled with human and chicken isolates. Our results indicate that is ubiquitous among Swedish corvid birds, with sporadic transmission to poultry and humans.
野生鸟类通过传播传染病对动物和人类健康构成潜在威胁。在本研究中，我们研究了瑞典鸦科鸟类中细菌性人畜共患病原体以及带有可转移抗菌耐药基因的肠杆菌的出现情况。对来自7个县14个地点的畜牧场附近的66只寒鸦、乌鸦、白嘴鸦和喜鹊的肠道进行了直接培养或PCR筛查，随后进行培养分析。通过全基因组测序对分离株进行了研究。在82%的鸟类中检测到了[具体细菌名称1]，在3%的鸟类中检测到了[具体细菌名称2]。在一个样本（2%）中发现了产超广谱β-内酰胺酶（ESBL）的[具体细菌名称3]，并携带[相关基因名称]。未鉴定出具有可转移碳青霉烯耐药性的[具体细菌名称4]。未发现[具体细菌名称5]或O157:H7，但对肠出血性[具体细菌名称6]毒力基因的PCR分析显示，35%的样本intimin呈阳性，9%的样本志贺毒素1呈阳性，17%的样本志贺毒素2呈阳性。通过基于基因组序列的多位点序列分型，将鸦科鸟类的[具体细菌名称7]分离株与之前发表的来自瑞典的分离株进行了比较。所有鸦科鸟类的[具体细菌名称7]分离株形成了一个簇，与人类和鸡的分离株混合在一起。我们的结果表明，[具体细菌名称7]在瑞典鸦科鸟类中普遍存在，偶尔会传播到家禽和人类身上。
