Wang Xiong, Zhang Lin, Zhang Lei, Wang Wenli, Wei Sihan, Wang Jie, Che Huilian, Zhang Yali
Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, College of Food Science and Nutritional Engineering, Beijing, China.
Genes Nutr. 2020 Jan 29;15:1. doi: 10.1186/s12263-020-0659-1. eCollection 2020.
Excessive intake of carbohydrates and fats causes over-nutrition, leading to a variety of diseases and complications. Here, we characterized the effects of different types of sugar and lipids on the growth and development of .
We measured the lifespan, reproductive capacity, and length of nematodes after sugars and lipids treatment alone and co-treatment of sugars and lipids. Furthermore, we studied the mechanisms underlying the damage caused by high-sucrose and high-stearic acid on by using transcriptome sequencing technology
The results showed that a certain concentration of sugar and lipid promoted the growth and development of nematodes. However, excessive sugars and lipids shortened the lifespan and length of nematodes and destroyed their reproductive capacity. Based on the results of the orthogonal test, we selected 400 mmol/L sucrose and 500 μg/mL stearic acid to model a high-sugar and high-lipid diet for .
High-sugar and high-lipid intake altered the expression of genes involved in biofilm synthesis, genes that catalyze the synthesis and degradation of endogenous substances, and genes involved in innate immunity, resulting in physiological damage. Furthermore, we explored the protective effect of resveratrol on high-sugar and high-lipid damage to nematodes. Resveratrol plays a role in repairing by participating in the metabolism of foreign substances and reducing cellular oxidative stress.
碳水化合物和脂肪的过量摄入会导致营养过剩，引发多种疾病和并发症。在此，我们描述了不同类型的糖和脂质对线虫生长发育的影响。
我们分别测量了单独用糖和脂质处理以及糖和脂质联合处理后线虫的寿命、繁殖能力和体长。此外，我们使用转录组测序技术研究了高糖和高硬脂酸对线虫造成损伤的潜在机制。
结果表明，一定浓度的糖和脂质促进了线虫的生长发育。然而，过量的糖和脂质缩短了线虫的寿命和体长，并破坏了它们的繁殖能力。基于正交试验的结果，我们选择400 mmol/L蔗糖和500 μg/mL硬脂酸来模拟线虫的高糖高脂饮食。
高糖高脂摄入改变了参与生物膜合成的基因、催化内源性物质合成和降解的基因以及参与先天免疫的基因的表达，导致生理损伤。此外，我们探索了白藜芦醇对高糖高脂对线虫损伤的保护作用。白藜芦醇通过参与外源物质的代谢和降低细胞氧化应激在修复中发挥作用。
