Department of Exercise Physiology, Kish International Campus (International Branch of University of Tehran), University of Tehran, Kish Island, Iran; Department of Education, Faculty of Social Science, Umeå University, Sweden; Department of Community Medicine and Rehabilitation, Section of Sports Medicine, Umeå University, Sweden.
Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran.
J Neuroimmunol. 2020 Apr 15;341:577172. doi: 10.1016/j.jneuroim.2020.577172. Epub 2020 Jan 30.
Multiple sclerosis (MS) is a neurodegenerative disease of the central nervous system. The animal model of MS, experimental autoimmune encephalomyelitis (EAE), is commonly used for studies of human inflammatory demyelinating diseases and has been shown to be suitable for studying the effects of exercise on MS pathophysiology. The present study was conducted to determine the impact of forced swimming and voluntary running wheel exercises before and after the induction of EAE on expression of Nogo-A, NgR, and ROCK genes in the brain tissue. A total of 96 C57BL/6 mice were randomly divided into two groups, namely exercises before (EXb, n = 48) and after (EXa, n = 48) induction of EAE. Each group was divided into four subgroups: Forced Swimming + EAE (n = 12), Voluntary Running Wheel + EAE (n = 12), NoEX-EAE (n = 12), and Control group (n = 12). Animals performed either swimming exercise for 30 min per day or running wheel for one hour per day, five days per week for four weeks. Results of Luxal Fast Blue (LFB) staining demonstrated that the degree of demyelination was significantly less in the experimental exercised compared to NoEX-EAE groups (P < .05). Amazingly, both modes of exercise reduced the severity of MS symptoms in mice exposed to swimming and wheel running, evaluated as body weight, clinical scores, degree of demyelination, and gene expressions, regardless of whether the exercise was performed before or after EAE induction.
多发性硬化症（MS）是一种中枢神经系统的神经退行性疾病。MS 的动物模型，实验性自身免疫性脑脊髓炎（EAE），常用于研究人类炎症性脱髓鞘疾病，并已被证明适合研究运动对 MS 病理生理学的影响。本研究旨在确定在 EAE 诱导前后进行强制游泳和自愿跑步轮运动对脑组织中 Nogo-A、NgR 和 ROCK 基因表达的影响。共有 96 只 C57BL/6 小鼠被随机分为两组，即 EAE 诱导前（EXb，n=48）和诱导后（EXa，n=48）运动组。每组分为四个亚组：强制游泳+EAE（n=12）、自愿跑步轮+EAE（n=12）、无 EX-EAE（n=12）和对照组（n=12）。动物每天进行 30 分钟的游泳运动或每天一小时的跑步轮运动，每周 5 天，持续 4 周。Luxal Fast Blue（LFB）染色结果表明，与 NoEX-EAE 组相比，实验组的脱髓鞘程度明显减轻（P<.05）。令人惊讶的是，无论运动是在 EAE 诱导前还是后进行，两种运动模式都能减轻暴露于游泳和轮跑中的小鼠的 MS 症状严重程度，评估指标为体重、临床评分、脱髓鞘程度和基因表达。
