Zhang Nan, Wei Shuxiang, Cao Shijie, Zhang Qiang, Kang Ning, Ding Liqin, Qiu Feng
School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Front Chem. 2020 Jan 23;7:908. doi: 10.3389/fchem.2019.00908. eCollection 2019.
Phytochemical investigation of Bge. var. (Hu et Fang) Fang obtained 33 triterpenoid saponins, including 14 new ones, aesculiside C-P (-). The structure elucidations were performed through comprehensive MS, 1D and 2D-NMR analysis, and their absolute configuration was unambiguously determined by X-ray diffraction analysis as well as Mo(OAc)-induced ECD method for the first time. All the substances were examined for their cytotoxic activities against three tumor cell lines, Hep G2, HCT-116, and MGC-803. Of these, compounds , , , , and exhibited potent cytotoxicities against all cell lines with IC of 2-21 μM, while compounds , , , , , , and depicted moderate activity (IC 13 to >40 μM). On these bases, the preliminary structure-activity correlations were also discussed. Meanwhile the neuroprotective properties of triterpenoid saponins from genus were evaluated for the first time. Among them, compounds , , , , , , , and exhibited moderate activities against CCl-induced PC12 cell injury.
对（胡先骕和方文培）方文培变种白背叶进行植物化学研究，得到了33种三萜皂苷，其中包括14种新的七叶皂苷C - P（-）。通过综合质谱、一维和二维核磁共振分析进行结构解析，并首次通过X射线衍射分析以及钼（乙酸盐）诱导的电子圆二色光谱法明确确定了它们的绝对构型。检测了所有物质对三种肿瘤细胞系Hep G2、HCT - 116和MGC - 803的细胞毒性活性。其中，化合物 、 、 、 和 对所有细胞系均表现出较强的细胞毒性，IC50为2 - 21 μM，而化合物 、 、 、 、 、 和 表现出中等活性（IC50为13至>40 μM）。在此基础上，还讨论了初步的构效关系。同时，首次评估了白背叶属三萜皂苷的神经保护特性。其中，化合物 、 、 、 、 、 、 和 对四氯化碳诱导的PC12细胞损伤表现出中等活性。
