Croatian Veterinary Institute, Veterinary Institute Križevci, Zakmardijeva 10, 48260 Križevci, Croatia.
Institute of Physics, Bijenička 46, 10000 Zagreb, Croatia.
Food Chem. 2020 Jun 30;316:126372. doi: 10.1016/j.foodchem.2020.126372. Epub 2020 Feb 6.
T-2 and its major metabolite HT-2 toxin are naturally occurring contaminants in cereals, with the highest concentrations determined in oats. Because of their toxicity and resistance to conventional methods used in mycotoxin degradation, development of new effective procedures for reduction of T-2/HT-2 toxin levels is needed. The aim of this study was to investigate the effect of different gas types within various timeframes on the efficiency of T-2 and HT-2 toxin degradation by low pressure dielectric barrier discharge (DBD) plasma in oat flour. Although humidity of the sample influences the intensity of the plasma, oxygen atoms efficiently oxidize the sample and produce CO and N. Before and after treatment, T-2 and HT-2 toxin concentrations were analysed by the confirmatory liquid chromatography tandem-mass spectrometry (LC-MS/MS) method. The highest reduction of T-2 and HT-2 toxin of 43.25% and 38.54%, respectively, was achieved using nitrogen plasma and this proved to be time-dependent.
T-2 及其主要代谢物 HT-2 毒素是谷物中天然存在的污染物，在燕麦中含量最高。由于它们的毒性和对霉菌毒素降解中常规方法的抗性，需要开发新的有效方法来降低 T-2/HT-2 毒素水平。本研究的目的是研究不同气体类型在不同时间内对低压介电阻挡放电（DBD）等离子体在燕麦粉中降解 T-2 和 HT-2 毒素效率的影响。尽管样品的湿度会影响等离子体的强度，但氧原子会有效地氧化样品并产生 CO 和 N。在处理前后，通过确证液相色谱串联质谱法（LC-MS/MS）分析 T-2 和 HT-2 毒素的浓度。使用氮气等离子体可分别实现 T-2 和 HT-2 毒素高达 43.25%和 38.54%的最高降解率，且这一效果具有时间依赖性。
