Chaiharn Mathurot, Theantana Teerayut, Pathom-Aree Wasu
Division of Biotechnology, Faculty of Science, Maejo University, Chiang Mai 50290, Thailand.
Department of Biology and Biotechnology, Faculty of Science and Technology, Nakhon Sawan Rajabhat University, Nakhon Sawan 60000, Thailand.
Pathogens. 2020 Feb 15;9(2):126. doi: 10.3390/pathogens9020126.
Rhizosphere bacteria can positively influence plant growth by direct and indirect mechanisms. A total of 112 bacterial strains were isolated from the rhizosphere of rice and tested for plant beneficial activities such as siderophore production, cell-wall-degrading enzyme production, hydrogen cyanide (HCN) production and antifungal activity against rice blast disease fungus. The actinomycetes count was 3.8 × 10 CFU/g soil. strains PC 12, D 4.1, D 4.3 and W1 showed strong growth inhibition of blast disease fungus, sp. (87.3%, 82.2%, 80.0% and 80.5%) in vitro. Greenhouse experiments revealed that rice plants treated with strain PC 12 recorded maximum plant height, root length and root dry weight compared to the control. Taxonomic characterization of this strain on the basis of 16S rRNA gene sequence led to its identification as PC 12. PC 12 may be used as biofertilizer to enhance the growth and productivity of commercially important rice cultivar RD6 and the biocontrol of blast disease fungus.
根际细菌可通过直接和间接机制对植物生长产生积极影响。从水稻根际分离出112株细菌菌株，并对其进行植物有益活性测试，如铁载体产生、细胞壁降解酶产生、氰化氢（HCN）产生以及对稻瘟病菌的抗真菌活性。放线菌数量为3.8×10 CFU/g土壤。PC 12、D 4.1、D 4.3和W1菌株在体外对稻瘟病菌（Magnaporthe oryzae）表现出强烈的生长抑制作用（分别为87.3%、82.2%、80.0%和80.5%）。温室试验表明，与对照相比，用PC 12菌株处理的水稻植株在株高、根长和根干重方面达到最大值。基于16S rRNA基因序列对该菌株进行分类学鉴定，结果将其鉴定为解淀粉芽孢杆菌（Bacillus amyloliquefaciens）PC 12。解淀粉芽孢杆菌PC 12可作为生物肥料，用于提高重要商业水稻品种RD6的生长和生产力，并对稻瘟病菌进行生物防治。
