Wegayehu Teklu, Li Junqiang, Karim Md Robiul, Zhang Longxian
College of Natural Sciences, Arba Minch University, Arba Minch, Ethiopia.
College of Animal Sciences and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.
Front Vet Sci. 2020 Jan 29;7:6. doi: 10.3389/fvets.2020.00006. eCollection 2020.
is the most frequently diagnosed microsporidian species in humans and occurs in a wide range of animals. This study was conducted in Central Ethiopia to determine the prevalence and genotypes of in lambs in order to evaluate their public health significance. Three hundred eighty nine fecal samples were collected and screened using a nested PCR targeting the internal transcribed spacer (ITS) of the ribosomal RNA gene. All positive PCR products were sequenced to determine the genotypes. was found in 39 (10.03%) of the lambs. Differences in the infection rates among sex and age groups were not significant ( > 0.05). Five ITS genotypes belonging to three known genotypes BEB6, COS-I, and COS-II, and two novel genotypes (ET-L1 and ET-L2) were identified in lambs. All five genotypes identified in the present study clustered within cattle-specific Group 2 in the ITS phylogenetic tree. This first molecular detection and characterization of in lambs in Ethiopia has identified the need for further studies in humans and other domestic animals in order to determine the public health significance of in Ethiopia.
是人类中最常被诊断出的微孢子虫物种，并且存在于多种动物中。本研究在埃塞俄比亚中部开展，以确定羔羊中该物种的流行率和基因型，从而评估其对公共卫生的重要性。收集了389份粪便样本，并使用针对核糖体RNA基因内部转录间隔区（ITS）的巢式PCR进行筛查。对所有阳性PCR产物进行测序以确定基因型。在39只（10.03%）羔羊中发现了该物种。性别和年龄组之间的感染率差异不显著（P>0.05）。在羔羊中鉴定出属于三种已知基因型BEB6、COS-I和COS-II以及两种新基因型（ET-L1和ET-L2）的五种ITS基因型。本研究中鉴定出的所有五种基因型在ITS系统发育树中都聚集在牛特异性第2组内。埃塞俄比亚首次对羔羊中的该物种进行分子检测和鉴定，表明有必要对人类和其他家畜开展进一步研究以确定该物种在埃塞俄比亚对公共卫生的重要性。
