He Fengyan, Li Mengyi, He Yi, Dong Zhe, Cao Jin, Dai Zhong, Ma Shuangcheng
National Institutes for Food and Drug Control, Beijing 100050, China.
J Anal Methods Chem. 2020 Feb 3;2020:8920380. doi: 10.1155/2020/8920380. eCollection 2020.
Processing of crude drug is a key character of traditional Chinese medicine (TCM), which could enhance the efficacy and/or reduce the toxicity of crude drugs to fulfill different requirements of TCM clinical practice. (EF) is a widely used TCM. As a traditional method of TCM, EF is processed with refined mutton fat before being used in clinical practice. It has been reported that processing EF with mutton fat could improve the bioavailability and intestinal absorption of epimedium flavonoids and thus enhances the pharmacological effects. For economic benefits, it is possible to adulterate processed EF with unprocessed drug or process EF with cheaper plant oils. In the present study, 17 batches of crude and processed EF samples were collected from the Chinese market and 10 batches of replica processed drugs were prepared with different edible plant oils and animal fats in our laboratory. Elemental analyzer coupled with isotopic ratio mass spectrometry (EA-IRMS) was applied to determine the C values of the cyclohexane extracts of those samples. Significant differences could be observed in the results. EA-IRMS could be used to discriminate raw EF, processed EF, and EF processed with C3 plant oils.
中药材炮制是中药的一个关键特性，它可以增强中药材的功效和/或降低其毒性，以满足中医临床实践的不同需求。淫羊藿是一种广泛使用的中药。作为一种传统的中药方法，淫羊藿在临床应用前要用精制羊脂进行炮制。据报道，用羊脂炮制淫羊藿可以提高淫羊藿黄酮的生物利用度和肠道吸收，从而增强药理作用。为了经济效益，有可能用未加工的药物掺假炮制后的淫羊藿，或用更便宜的植物油炮制淫羊藿。在本研究中，从中国市场收集了17批生淫羊藿和炮制淫羊藿样品，并在我们实验室用不同的食用植物油和动物脂肪制备了10批仿制炮制药物。采用元素分析仪结合同位素比值质谱仪（EA-IRMS）测定这些样品环己烷提取物的碳值。结果可观察到显著差异。EA-IRMS可用于区分生淫羊藿、炮制淫羊藿和用C3植物油炮制的淫羊藿。
