Jiangsu Key Laboratory of Bioactive Natural Product Research and State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
Jiangsu Key Laboratory of Bioactive Natural Product Research and State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
Fitoterapia. 2020 Apr;142:104518. doi: 10.1016/j.fitote.2020.104518. Epub 2020 Feb 22.
Aphanamoxene A-D (1-4), three new acyclic diterpene derivatives and one new acyclic norsesquiterpene were isolated from the seed of Aphanamixis polystachya. Their structures were elucidated on the basis of extensive spectroscopic methods, including 1D and 2D NMR and HRESIMS. And the absolute configuration of 1 was achieved by Mosher method. These acyclic terpenoids (1-4) showed obvious nitric oxide production inhibitory activity on lipopolysaccharide-Induced RAW264.7 macrophages with IC values of 17.6 ± 1.4, 9.8 ± 0.7, 16.6 ± 1.2, and 14.2 ± 0.9 μM, respectively.
从密花罗锅底种子中分离得到了四个新的非环二萜衍生物和一个新的非环倍半萜，分别命名为 Aphanamoxene A-D (1-4)。通过 1D 和 2D NMR 以及 HRESIMS 等多种波谱方法解析了它们的结构，并通过 Mosher 方法确定了 1 的绝对构型。这些非环萜类化合物（1-4）对脂多糖诱导的 RAW264.7 巨噬细胞产生的一氧化氮具有明显的抑制作用，IC50 值分别为 17.6±1.4、9.8±0.7、16.6±1.2 和 14.2±0.9 μM。
