Kazimirova Alena, El Yamani Naouale, Rubio Laura, García-Rodríguez Alba, Barancokova Magdalena, Marcos Ricard, Dusinska Maria
Department of Biology, Faculty of Medicine, Slovak Medical University, 833 03 Bratislava, Slovakia.
Health Effects Laboratory, Department of Environmental Chemistry, NILU-Norwegian Institute for Air Research, N-2027 Kjeller, Norway.
Nanomaterials (Basel). 2020 Mar 5;10(3):465. doi: 10.3390/nano10030465.
The genotoxicity of anatase/rutile TiO nanoparticles (TiO NPs, NM105 at 3, 15 and 75 µg/cm) was assessed with the mammalian in-vitro Hypoxanthine guanine phosphoribosyl transferase () gene mutation test in Chinese hamster lung (V79) fibroblasts after 24 h exposure. Two dispersion procedures giving different size distribution and dispersion stability were used to investigate whether the effects of TiO NPs depend on the state of agglomeration. TiO NPs were fully characterised in the previous European FP7 projects NanoTEST and NanoREG2. Uptake of TiO NPs was measured by transmission electron microscopy (TEM). TiO NPs were found in cytoplasmic vesicles, as well as close to the nucleus. The internalisation of TiO NPs did not depend on the state of agglomeration and dispersion used. The cytotoxicity of TiO NPs was measured by determining both the relative growth activity (RGA) and the plating efficiency (PE). There were no substantial effects of exposure time (24, 48 and 72 h), although a tendency to lower RGA at longer exposure was observed. No significant difference in PE values and no increases in the gene mutant frequency were found in exposed relative to unexposed cultures in spite of evidence of uptake of NPs by cells.
在24小时暴露后，通过中国仓鼠肺（V79）成纤维细胞中的哺乳动物体外次黄嘌呤鸟嘌呤磷酸核糖基转移酶（）基因突变试验，评估了锐钛矿/金红石型二氧化钛纳米颗粒（TiO NPs，NM105，浓度为3、15和75μg/cm）的遗传毒性。使用两种产生不同尺寸分布和分散稳定性的分散程序，来研究TiO NPs的效应是否取决于团聚状态。TiO NPs在之前的欧洲FP7项目NanoTEST和NanoREG2中已得到充分表征。通过透射电子显微镜（TEM）测量TiO NPs的摄取情况。在细胞质囊泡以及靠近细胞核的位置发现了TiO NPs。TiO NPs的内化不取决于所使用的团聚和分散状态。通过测定相对生长活性（RGA）和接种效率（PE）来测量TiO NPs的细胞毒性。尽管观察到较长暴露时间有降低RGA的趋势，但暴露时间（24、48和72小时）没有实质性影响。尽管有细胞摄取NP的证据，但在暴露培养物与未暴露培养物中，未发现PE值有显著差异，也未发现基因突变速率增加。
