Division of Biological Science and Technology, Yonsei University, Wonju 26493, Republic of Korea.
Department of Biochemistry, Kangwon National University, Chuncheon 24341, Republic of Korea.
J Microbiol Biotechnol. 2020 Jul 28;30(7):1044-1050. doi: 10.4014/jmb.2002.02024.
(Linn.) is a medicinal herbal plant that is commonly used to treat chronic kidney disease and hepatitis. However, its effect on cell proliferation has not been clearly revealed. In this report, we sought to determine the effect of the flower extract of (FA) on cell proliferation. Based on our findings, FA increased the proliferation of human diploid fibroblast (HDF) and HEK293 cells. Through cell cycle analysis, FA was found to increase the number of HDF cells in the S phase and G2/M phase. FA also increased the expression of cyclin D1 and enhanced the migration of HDF cells. By administering FA to HDF cells with ≥30 passages, a decrease in the number of senescence-associated β galactosidase-positive cells was observed, thereby indicating that FA can ameliorate cellular senescence. Collectively, our findings indicate that FA increases cyclin D1 expression and regulates cell proliferation.
（ Linn. ）是一种药用草本植物，常用于治疗慢性肾病和肝炎。然而，其对细胞增殖的影响尚未明确揭示。在本报告中，我们旨在确定花提取物（FA）对细胞增殖的影响。根据我们的发现，FA 可增加人二倍体成纤维细胞（HDF）和 HEK293 细胞的增殖。通过细胞周期分析，FA 被发现可增加 HDF 细胞在 S 期和 G2/M 期的数量。FA 还增加了细胞周期蛋白 D1 的表达并增强了 HDF 细胞的迁移。通过向具有 ≥30 个传代的 HDF 细胞给予 FA，观察到衰老相关β半乳糖苷酶阳性细胞的数量减少，这表明 FA 可以改善细胞衰老。总之，我们的研究结果表明，FA 可增加 cyclin D1 的表达并调节细胞增殖。
