School of Medical Science, Gold Coast campus, Griffith University, Southport QLD 4222, Australia.
Université de Strasbourg, CNRS, Génétique Moléculaire Génomique Microbiologie GMGM UMR 7156, F-67000 Strasbourg, France.
Cells. 2020 Mar 10;9(3):672. doi: 10.3390/cells9030672.
The budding yeast has an actin cytoskeleton that comprises a set of protein components analogous to those found in the actin cytoskeletons of higher eukaryotes. Furthermore, the actin cytoskeletons of and of higher eukaryotes have some similar physiological roles. The genetic tractability of budding yeast and the availability of a stable haploid cell type facilitates the application of molecular genetic approaches to assign functions to the various actin cytoskeleton components. This has provided information that is in general complementary to that provided by studies of the equivalent proteins of higher eukaryotes and hence has enabled a more complete view of the role of these proteins. Several human functional homologues of yeast actin effectors are implicated in diseases. A better understanding of the molecular mechanisms underpinning the functions of these proteins is critical to develop improved therapeutic strategies. In this article we chose as examples four evolutionarily conserved proteins that associate with the actin cytoskeleton: 1) yeast Hof1p/mammalian PSTPIP1, 2) yeast Rvs167p/mammalian BIN1, 3) yeast eEF1A/eEF1A1 and eEF1A2 and 4) yeast Yih1p/mammalian IMPACT. We compare the knowledge on the functions of these actin cytoskeleton-associated proteins that has arisen from studies of their homologues in yeast with information that has been obtained from in vivo studies using live animals or in vitro studies using cultured animal cell lines.
芽殖酵母具有由一组蛋白成分组成的肌动蛋白细胞骨架，这些蛋白成分类似于高等真核生物肌动蛋白细胞骨架中的蛋白成分。此外，芽殖酵母和高等真核生物的肌动蛋白细胞骨架具有一些相似的生理作用。芽殖酵母的遗传可操作性和稳定的单倍体细胞类型的可用性，促进了分子遗传学方法的应用，以赋予各种肌动蛋白细胞骨架成分功能。这提供了与高等真核生物等效蛋白研究提供的信息通常互补的信息，从而使人们能够更全面地了解这些蛋白的作用。几种人类功能性酵母肌动蛋白效应物同源物与疾病有关。更好地了解这些蛋白功能的分子机制对于开发改进的治疗策略至关重要。在本文中，我们选择了与肌动蛋白细胞骨架相关的四个进化上保守的蛋白作为示例：1) 酵母 Hof1p/哺乳动物 PSTPIP1，2) 酵母 Rvs167p/哺乳动物 BIN1，3) 酵母 eEF1A/eEF1A1 和 eEF1A2，以及 4) 酵母 Yih1p/哺乳动物 IMPACT。我们将从酵母同源物研究中获得的关于这些与肌动蛋白细胞骨架相关的蛋白功能的知识与使用活体动物进行的体内研究或使用培养的动物细胞系进行的体外研究获得的信息进行了比较。
