State Key Laboratory of Urban Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, 150001, Harbin, China.
Condensed Matter Science and Technology Institute, School of Instrumentation Science and Engineering, Harbin Institute of Technology, 150001, Harbin, China.
Nat Commun. 2020 Mar 13;11(1):1379. doi: 10.1038/s41467-020-14866-0.
Bioelectricity generation, by Shewanella oneidensis (S. oneidensis) MR-1, has become particularly alluring, thanks to its extraordinary prospects for energy production, pollution treatment, and biosynthesis. Attempts to improve its technological output by modification of S. oneidensis MR-1 remains complicated, expensive and inefficient. Herein, we report on the augmentation of S. oneidensis MR-1 with carbon dots (CDs). The CDs-fed cells show accelerated extracellular electron transfer and metabolic rate, with increased intracellular charge, higher adenosine triphosphate level, quicker substrate consumption and more abundant extracellular secretion. Meanwhile, the CDs promote cellular adhesion, electronegativity, and biofilm formation. In bioelectrical systems the CDs-fed cells increase the maximum current value, 7.34 fold, and power output, 6.46 fold. The enhancement efficacy is found to be strongly dependent on the surface charge of the CDs. This work demonstrates a simple, cost-effective and efficient route to improve bioelectricity generation of S. oneidensis MR-1, holding promise in all relevant technologies.
得益于其在能源生产、污染处理和生物合成方面的非凡前景，希瓦氏菌（Shewanella oneidensis）MR-1 的生物电能产生已经变得特别有吸引力。通过修饰 S. oneidensis MR-1 来提高其技术输出的尝试仍然很复杂、昂贵且效率低下。在此，我们报告了用碳点 (CDs) 来增强 S. oneidensis MR-1。与对照组相比，CDs 喂养的细胞表现出更快的细胞外电子转移和代谢率，细胞内电荷增加，三磷酸腺苷水平更高，底物消耗更快，细胞外分泌更丰富。同时，CDs 促进细胞黏附、电负性和生物膜形成。在生物电化学系统中，CDs 喂养的细胞将最大电流值提高了 7.34 倍，将功率输出提高了 6.46 倍。增强效果强烈依赖于 CDs 的表面电荷。这项工作展示了一种简单、经济高效的方法来提高 S. oneidensis MR-1 的生物电能产生，有望在所有相关技术中得到应用。
