Chemical Composition of an Aphid Antifeedant Extract from an Endophytic Fungus, sp. EFI671.

Botanical and fungal biopesticides, including endophytes, are in high demand given the current restrictive legislations on the use of chemical pesticides. As part of an ongoing search for new biopesticides, a series of fungal endophytes have been isolated from selected medicinal plants including species. In the current study, an extract from the endophytic fungus sp. EFI 671, isolated from the stem parts of the medicinal plant sp., was screened for bioactivity against plant pathogens (, , and ), insect pests (, , ) and plant parasites (), with positive results against . The chemical study of the neutral fraction of the active hexane extract resulted in the isolation of a triglyceride mixture (m), eburicol (), β-sitostenone (), ergosterol () and ergosterol peroxide (). The free fatty acids present in the acid fraction of the extract and in m1 (oleic, linoleic, palmitic and stearic) showed strong dose-dependent antifeedant effects against . Liquid (potato dextrose broth, PDB and Sabouraud Broth, SDB) and solid (corn, sorghum, pearl millet and rice) growth media were tested in order to optimize the yield and bioactivity of the fungal extracts. Pearl millet and corn gave the highest extract yields. All the extracts from these solid media had strong effects against , with sorghum being the most active. Corn media increased the methyl linoleate content of the extract, pearl millet media increased the oleic acid and sorghum media increased the oleic and linoleic acids compared to rice. The antifeedant effects of these extracts correlated with their content in methyl linoleate and linoleic acid. The phytotoxic effects of these extracts against ryegrass, , and lettuce, , varied with culture media, with sorghum being non- toxic.