柏科植物土木香内酯和二萜的细胞毒性和抗炎作用。

Cytotoxic and anti-inflammatory effects of lignans and diterpenes from Cupressus macrocarpa.

机构信息

Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo 11566, Egypt.

Graduate Institute of Natural Products, Center for Natural Product Research and Development, College of Pharmacy, Drug Development and Value Creation Research Center, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.

出版信息

Bioorg Med Chem Lett. 2020 May 15;30(10):127127. doi: 10.1016/j.bmcl.2020.127127. Epub 2020 Mar 20.

DOI:10.1016/j.bmcl.2020.127127

PMID:32223924

Abstract

Cupressus macrocarpa is a windbreak tree and is reported to have various cytotoxic effects. A natural product study on the leaves of C. macrocarpa has yielded ten secondary metabolites, including three new diterpenoids (1-3), four known diterpenoids (4-7), and three known lignans (8-10). The structures of all isolated compounds were elucidated via the interpretation of spectroscopic methods, especially 2D NMR and mass analyses. In the cytotoxic assays, compounds 1-3 and 7-10 showed inhibition effect against HepG2, MDA-MB-231, and A549 cells with IC values ranging from 0.004 to 19.9 μg/mL. Moreover, the anti-inflammatory assays revealed that (-)-matairesinol (8) had significant inhibitory activities on superoxide anion generation (IC = 2.7 ± 0.3 μM) and elastase release (IC = 6.6 ± 0.7 μM).

摘要

圆柏是一种防风林树种，据报道具有多种细胞毒性作用。对圆柏叶的天然产物研究产生了十个次生代谢产物，包括三个新的二萜（1-3），四个已知的二萜（4-7）和三个已知的木脂素（8-10）。通过对光谱方法，特别是二维 NMR 和质谱分析的解释，阐明了所有分离化合物的结构。在细胞毒性测定中，化合物 1-3 和 7-10 对 HepG2、MDA-MB-231 和 A549 细胞显示出抑制作用，IC 值范围为 0.004 至 19.9μg/mL。此外，抗炎测定表明 (-)-matairesinol（8）对超氧阴离子生成（IC=2.7±0.3μM）和弹性蛋白酶释放（IC=6.6±0.7μM）具有显著的抑制活性。