Zhou Qiulian, Shi Chao, Lv Yicheng, Zhao Chenglin, Jiao Zheng, Wang Tianhui
Shanghai Applied Radiation Institute, School of Environmental and Chemical Engineering, Shanghai University, Shanghai, China.
Cardiac Regeneration and Ageing Lab, Institute of Cardiovascular Sciences, School of Life Sciences, Shanghai University, Shanghai, China.
Front Genet. 2020 Mar 18;11:256. doi: 10.3389/fgene.2020.00256. eCollection 2020.
Circulating microRNAs (miRNAs, miRs) have great potential as cardiac biomarkers and they are also being explored for their roles in intercellular communication and gene expression regulation. The analysis of circulating miRNAs in response to exercise would provide a deeper understanding of the molecular response to physical activity and valuable information for clinical practice. Here, eight male college students were recruited to participate in cardiopulmonary exercise testing (CPET) and 1 h acute exercise training (AET). Blood samples were collected and serum miRNAs involved in angiogenesis, inflammation and enriched in muscle and/or cardiac tissues were analyzed before and after cardiopulmonary exercise and acute exercise. The miRNAs we detected were miR-1, miR-20a, miR-21, miR-126, miR-133a, miR-133b, miR-146, miR155, miR-208a, miR-208b, miR-210, miR-221, miR-222, miR-328, miR-378, miR-499, and miR-940. We found that serum miR-20a was decreased significantly after CPET and serum miR-21 was increased after AET. In addition, no robust correlation was identified between the changes of these miRNAs and makers of cardiac function and exercise capacity, which indicates a distinct adaptation of these miRNAs to exercise. Future studies are highly needed to define the potential use of these circulating miRNAs as useful biomarkers of exercise training, and disclose the biological function of circulating miRNAs as physiological mediators of exercise-induced cardiovascular adaptation.
循环微RNA（miRNA，miR）作为心脏生物标志物具有巨大潜力，人们也在探索它们在细胞间通讯和基因表达调控中的作用。分析运动后循环miRNA将有助于更深入地了解身体活动的分子反应，并为临床实践提供有价值的信息。在此，招募了8名男性大学生参与心肺运动测试（CPET）和1小时急性运动训练（AET）。在心肺运动和急性运动前后采集血样，分析参与血管生成、炎症且在肌肉和/或心脏组织中富集的血清miRNA。我们检测的miRNA有miR-1、miR-20a、miR-21、miR-126、miR-133a、miR-133b、miR-146、miR155、miR-208a、miR-208b、miR-210、miR-221、miR-222、miR-328、miR-378、miR-499和miR-940。我们发现CPET后血清miR-20a显著降低，AET后血清miR-21升高。此外，未发现这些miRNA的变化与心脏功能和运动能力指标之间存在强相关性，这表明这些miRNA对运动有独特的适应性。未来迫切需要开展研究来确定这些循环miRNA作为运动训练有用生物标志物的潜在用途，并揭示循环miRNA作为运动诱导心血管适应生理介质的生物学功能。
