中国广东携带 Kobuvirus 的褐家鼠。
Kobuviruses carried by Rattus norvegicus in Guangdong, China.
机构信息
Department of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical University, 1838 North Road Guangzhou, Guangzhou, 510515, China.
出版信息
BMC Microbiol. 2020 Apr 15;20(1):94. doi: 10.1186/s12866-020-01767-x.
BACKGROUND
Murine kobuviruses (MuKV) are newly recognized picornaviruses first detected in murine rodents in the USA in 2011. Little information on MuKV epidemiology in murine rodents is available. Therefore, we conducted a survey of the prevalence and genomic characteristics of rat kobuvirus in Guangdong, China.
RESULTS
Fecal samples from 223 rats (Rattus norvegicus) were collected from Guangdong and kobuviruses were detected in 12.6% (28) of samples. Phylogenetic analysis based on partial 3D and complete VP1 sequence regions showed that rat kobuvirus obtained in this study were genetically closely related to those of rat/mouse kobuvirus reported in other geographical areas. Two near full-length rat kobuvirus genomes (MM33, GZ85) were acquired and phylogenetic analysis of these revealed that they shared very high nucleotide/amino acids identity with one another (95.4%/99.4%) and a sewage-derived sequence (86.9%/93.5% and 87.5%/93.7%, respectively). Comparison with original Aichivirus A strains, such human kobuvirus, revealed amino acid identity values of approximately 80%.
CONCLUSION
Our findings indicate that rat kobuvirus have distinctive genetic characteristics from other Aichivirus A viruses. Additionally, rat kobuvirus may spread via sewage.
背景
鼠诺如病毒（MuKV）是一种新发现的小核糖核酸病毒，于 2011 年首次在美国啮齿动物鼠中被发现。目前有关鼠诺如病毒在啮齿动物中的流行情况的信息较少。因此，我们在中国广东进行了一项关于大鼠诺如病毒流行情况和基因组特征的调查。
结果
从广东采集了 223 只大鼠（Rattus norvegicus）的粪便样本，其中 12.6%（28 个）样本检测到了诺如病毒。基于部分 3D 和完整 VP1 序列区的系统进化分析表明，本研究中获得的大鼠诺如病毒在遗传上与其他地理区域报告的大鼠/小鼠诺如病毒密切相关。获得了两个近乎全长的大鼠诺如病毒基因组（MM33、GZ85），对这些基因组的系统进化分析表明，它们彼此之间具有非常高的核苷酸/氨基酸同一性（95.4%/99.4%和 87.5%/93.7%），与污水衍生序列的核苷酸/氨基酸同一性分别为 86.9%/93.5%和 87.5%/93.7%。与其他 Aichivirus A 病毒（如人类诺如病毒）相比，比较发现其氨基酸同一性值约为 80%。
结论
我们的研究结果表明，大鼠诺如病毒具有与其他 Aichivirus A 病毒不同的遗传特征。此外，大鼠诺如病毒可能通过污水传播。
Zotero 插件