Nasioutziki Maria, Chatzistamatiou Kimon, Loufopoulos Panagiotis-Dimitrios, Vavoulidis Eleftherios, Tsampazis Nikolaos, Pratilas George-Chrysostomos, Liberis Anastasios, Karpa Vasiliki, Parcharidis Evanggelos, Daniilidis Angelos, Spanos Konstantinos, Dinas Konstantinos
12nd Obstetrics & Gynaecology Department, Molecular & Morphological Clinical Cytopathology Laboratory, Hippokration General Hospital, Medical Faculty, School of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.
21st Department of General Surgery, Anal & Colorectal Clinic, Papageorgiou General Hospital, Medical Faculty, School of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Infect Agent Cancer. 2020 Apr 15;15:22. doi: 10.1186/s13027-020-00287-7. eCollection 2020.
Infection with human papillomaviruses (HPVs) can cause benign and malignant tumours in the anogenital tract and the oropharynx both in men and women. The aim of the presented study was to investigate cervical, anal, and oral HPV-detection rates among women referred to colposcopy for abnormal Cervical Cancer (CaCx) screening results and assess the concordance of HPV-types among these anatomical sites.
Women referred to colposcopy at a single centre due to abnormal cytology, conducted for CaCx screening, were subjected to cervical Liquid-based Cytology (LBC) smear testing, anal and oral sampling. Routine colposcopy consisted in multiple biopsies and/or Endocervical Curettage (ECC). HPV-detection was performed by PCR genotyping in all three anatomical sites. In high-risk (hr) HPV-DNA positive samples either from anal canal or oral cavity, anal LBC cytology and anoscopy were performed, or oral cavity examination respectively. Descriptive statistics was used for the analysis of HPV-detection rates and phi-coefficient for the determination of HPV-positivity concordance between the anatomical sites.
Out of 118 referred women, hr. HPV-DNA was detected in 65 (55.1%), 64 (54.2%) and 3 (2.5%) at cervix, anal canal and oral cavity respectively while low-risk HPV-DNA was detected in 14 (11.9%) and 11 (9.3%) at cervix and anal canal respectively. The phi-coefficient for cervix/anal canal was 0.392 for HPV16, 0.658 for HPV31, 0.758 for HPV33, - 0.12 for HPV45, 0.415 for HPV52 and 0.473 for HPV58. All values were statistically significant ( < 0.001).
The results suggest that most HPV-types, high-risk and low-risk, detected in the cervix of women with prevalent cervical dysplasia, correlate with the ones detected in their anal canal. This particularly applies for the HPV-types included in the nonavalent HPV-vaccine (HPVs 6/11/16/18/31/33/45/52/58).
人乳头瘤病毒（HPV）感染可导致男性和女性的肛门生殖道及口咽出现良性和恶性肿瘤。本研究的目的是调查因宫颈癌（CaCx）筛查结果异常而转诊至阴道镜检查的女性中宫颈、肛门和口腔的HPV检测率，并评估这些解剖部位之间HPV型别的一致性。
因细胞学异常转诊至单一中心进行CaCx筛查的女性，接受宫颈液基细胞学（LBC）涂片检测、肛门和口腔采样。常规阴道镜检查包括多次活检和/或宫颈管刮除术（ECC）。在所有三个解剖部位通过PCR基因分型进行HPV检测。对于来自肛管或口腔的高危（hr）HPV-DNA阳性样本，分别进行肛门LBC细胞学检查和肛门镜检查或口腔检查。描述性统计用于分析HPV检测率，phi系数用于确定解剖部位之间HPV阳性的一致性。
在118名转诊女性中，分别在宫颈、肛管和口腔检测到hr.HPV-DNA的有65例（55.1%）、64例（54.2%）和3例（2.5%），而在宫颈和肛管分别检测到低危HPV-DNA的有14例（11.9%）和11例（9.3%）。宫颈/肛管的phi系数，HPV16为0.392，HPV31为0.658，HPV33为0.758，HPV45为-0.12，HPV52为0.415，HPV58为0.473。所有值均具有统计学意义（<0.001）。
结果表明，在宫颈发育异常普遍的女性宫颈中检测到的大多数HPV型别，无论是高危型还是低危型，都与在其肛管中检测到的型别相关。这尤其适用于九价HPV疫苗所包含的HPV型别（HPV 6/11/16/18/31/33/45/52/58）。
