用于分离染色体重复间重组增强的大肠杆菌突变体的方法。
Method for the isolation of Escherichia coli mutants with enhanced recombination between chromosomal duplications.
作者信息
Konrad E B
出版信息
J Bacteriol. 1977 Apr;130(1):167-72. doi: 10.1128/jb.130.1.167-172.1977.
Abstract
A method is described for the isolation of Escherichia coli mutants that show increased recombination between a pair of chromosomal duplications. These "hyper-rec" mutants display a variety of secondary phenotypes. I have isolated a large number of hyper-rec mutants and found them useful in screening for mutants that accumulate labeled DNA fragments after short pulses with [3H]thymidine. The mutants so recovered include ones that are defective in deoxyribonucleic acid ligase, deoxyribonucleic acid polymerase I and its associated 5' yields 3' exonuclease, and a group of mutants, dnaS, that accumulate abnormally short Okazaki fragments. Evidence is presented that suggests that the lac-att80 segment of the chromosome cannot be inverted.
摘要
本文描述了一种分离大肠杆菌突变体的方法，这些突变体在一对染色体重复序列之间表现出增加的重组。这些“高重组”突变体表现出多种次级表型。我已经分离出大量的高重组突变体，并发现它们可用于筛选在用[3H]胸苷短脉冲处理后积累标记DNA片段的突变体。如此回收的突变体包括那些在脱氧核糖核酸连接酶、脱氧核糖核酸聚合酶I及其相关的5'→3'核酸外切酶中存在缺陷的突变体，以及一组积累异常短冈崎片段的dnaS突变体。有证据表明染色体的lac-att80区段不能倒转。
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本文引用的文献
1
A DELETION ANALYSIS OF THE LAC OPERATOR REGION IN ESCHERICHIA COLI.大肠杆菌中乳糖操纵子区域的缺失分析
J Mol Biol. 1964 Mar;8:427-30. doi: 10.1016/s0022-2836(64)80206-0.
2
Genetic regulatory mechanisms in the synthesis of proteins.蛋白质合成中的遗传调控机制。
J Mol Biol. 1961 Jun;3:318-56. doi: 10.1016/s0022-2836(61)80072-7.
3
Slow joining of newly replicated DNA chains in DNA polymerase I-deficient Escherichia coli mutants.DNA聚合酶I缺陷型大肠杆菌突变体中新复制DNA链的缓慢连接。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):2954-7. doi: 10.1073/pnas.68.12.2954.
4
A possible function of DNA polymerase in chromosome replication.DNA聚合酶在染色体复制中的一种可能功能。
Biochem Biophys Res Commun. 1970 Nov 25;41(4):973-80. doi: 10.1016/0006-291x(70)90180-4.
5
Enzymatic joining of polynucleotides. IX. A simple and rapid assay of polynucleotide joining (ligase) activity by measurement of circle formation from linear deoxyadenylate-deoxythymidylate copolymer.多核苷酸的酶促连接。IX. 通过测量线性脱氧腺苷酸-脱氧胸苷酸共聚物形成环来简单快速地测定多核苷酸连接（连接酶）活性。
J Biol Chem. 1970 Jul 25;245(14):3626-31.
6
Properties of a temperature-sensitive, radiation-sensitive mutant of Escherichia coli. II. DNA replication.大肠杆菌温度敏感、辐射敏感突变体的特性。II. DNA复制
Proc Natl Acad Sci U S A. 1969 Dec;64(4):1195-202. doi: 10.1073/pnas.64.4.1195.
7
Isolation of an E. coli strain with a mutation affecting DNA polymerase.分离出一株具有影响DNA聚合酶突变的大肠杆菌菌株。
Nature. 1969 Dec 20;224(5225):1164-6. doi: 10.1038/2241164a0.
8
Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division.在DNA合成和细胞分裂过程中受到影响的大肠杆菌热敏突变体。
Cold Spring Harb Symp Quant Biol. 1968;33:677-93. doi: 10.1101/sqb.1968.033.01.077.
9
Properties of a temperature-sensitive radiation-sensitive mutant of Escherichia coli.大肠杆菌温度敏感辐射敏感突变体的特性
Proc Natl Acad Sci U S A. 1968 Aug;60(4):1495-502. doi: 10.1073/pnas.60.4.1495.
10
Replication of Escherichia coli requires DNA polymerase I.大肠杆菌的复制需要DNA聚合酶I。
Nature. 1974 Aug 9;250(5466):513-4. doi: 10.1038/250513a0.
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