Konkel Michael E, Talukdar Prabhat K, Negretti Nicholas M, Klappenbach Courtney M
School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, WA, United States.
Front Microbiol. 2020 Apr 9;11:564. doi: 10.3389/fmicb.2020.00564. eCollection 2020.
, a foodborne pathogen, is one of the most common bacterial causes of gastroenteritis in the world. Undercooked poultry, raw (unpasteurized) dairy products, untreated water, and contaminated produce are the most common sources associated with infection. establishes a niche in the gut by adhering to and invading epithelial cells, which results in diarrhea with blood and mucus in the stool. The process of colonization is mediated, in part, by surface-exposed molecules (adhesins) that bind directly to host cell ligands or the extracellular matrix (ECM) surrounding cells. In this review, we introduce the known and putative adhesins of the foodborne pathogen We then focus our discussion on two Microbial Surface Components Recognizing Adhesive Matrix Molecule(s) (MSCRAMMs), termed CadF and FlpA, which have been demonstrated to contribute to colonization and pathogenesis. studies have determined that these two surface-exposed proteins bind to the ECM glycoprotein fibronectin (FN). studies have shown that and mutants exhibit impaired colonization of chickens compared to the wild-type strain. Additional studies have revealed that CadF and FlpA stimulate epithelial cell signaling pathways necessary for cell invasion. Interestingly, CadF and FlpA have distinct FN-binding domains, suggesting that the functions of these proteins are non-redundant. In summary, the binding of FN by CadF and FlpA adhesins has been demonstrated to contribute to adherence, invasion, and cell signaling.
一种食源性病原体，是世界上引起肠胃炎最常见的细菌原因之一。未煮熟的家禽、生（未巴氏杀菌）乳制品、未经处理的水和受污染的农产品是与感染相关的最常见来源。通过粘附和侵入上皮细胞在肠道中建立生态位，这会导致粪便中带血和粘液的腹泻。定植过程部分由直接结合宿主细胞配体或细胞周围细胞外基质（ECM）的表面暴露分子（粘附素）介导。在本综述中，我们介绍了这种食源性病原体已知的和推测的粘附素。然后我们将讨论重点放在两种微生物表面成分识别粘附基质分子（MSCRAMMs）上，称为CadF和FlpA，它们已被证明有助于该病原体的定植和发病机制。研究已确定这两种表面暴露蛋白与ECM糖蛋白纤连蛋白（FN）结合。研究表明，与野生型菌株相比，CadF和FlpA突变体在鸡体内的定植受损。其他研究表明，CadF和FlpA刺激细胞入侵所需的上皮细胞信号通路。有趣的是，CadF和FlpA具有不同的FN结合结构域，这表明这些蛋白质的功能并非冗余。总之，已证明该病原体的CadF和FlpA粘附素与FN的结合有助于粘附、入侵和细胞信号传导。
